MOPS
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- This page is about the biochemical buffering agent. For information about other types of mops, see Mops (disambiguation).
| MOPS | |
|---|---|
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| IUPAC name | 3-morpholinopropane-1-sulfonic acid |
| Other names | 3-(N-morpholino)propanesulfonic acid |
| Identifiers | |
| CAS number | [1132-61-2] |
| PubChem | |
| SMILES | C1COCCN1CCCS(=O)(=O)O |
| Properties | |
| Molecular formula | C7H15NO4S |
| Molar mass | 209.2633 |
| Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa) Infobox disclaimer and references |
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MOPS is the common name for the compound 3-(N-morpholino)propanesulfonic acid a buffer introduced by Good et al. in the 1960's. It is a structural analog to MES.[1] Its chemical structure contains a morpholine ring. HEPES is a similar pH buffering compound which contains a piperazine ring. With a pKa of 7.20, MOPS is an excellent buffer for many biological systems at near-neutral pH.
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[edit] Applications
MOPS is frequently used as a buffering agent in biology and biochemistry. It is has been tested and recommended for polyacrylamide gel electrophoresis.[2]Usage above 20mM in mammalian cell culture work is not recommended.[3]
[edit] References
- ^ Good, N.E. et al., Biochemistry, 5, 467-477 (1966).
- ^ Thomas, J.M. and Hodes, M.E., Analytical Biochemistry, 118, 194-196 (1981).
- ^ Eagle, H., Science, 174, 500-503 (1971).
[edit] See also
[edit] External links
- Safety information
- Sigma Aldrich Buffer Calculator - Useful tool to calculate weight, volume, or concentration from molecular weight.
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