Methylmalonate-semialdehyde dehydrogenase (acylating)

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In enzymology, a methylmalonate-semialdehyde dehydrogenase (acylating) (EC 1.2.1.27) is an enzyme that catalyzes the chemical reaction

2-methyl-3-oxopropanoate + CoA + H₂O + NAD⁺ propanoyl-CoA + HCO₃- + NADH

The 4 substrates of this enzyme are 2-methyl-3-oxopropanoate, CoA, H₂O, and NAD⁺, whereas its 3 products are propanoyl-CoA, HCO3-, and NADH.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the aldehyde or oxo group of donor with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is 2-methyl-3-oxopropanoate:NAD+ 3-oxidoreductase (CoA-propanoylating). Other names in common use include MSDH, and MMSA dehydrogenase. This enzyme participates in 3 metabolic pathways: inositol metabolism, valine, leucine and isoleucine degradation, and propanoate metabolism.

Contents 1 Structural studies 2 References 3 External links 3.1 Gene Ontology (GO) codes

[edit] Structural studies

As of late 2007, only one structure has been solved for this class of enzymes, with the PDB accession code 1T90.

[edit] References

• IUBMB entry for 1.2.1.27
• BRENDA references for 1.2.1.27 (Recommended.)
• PubMed references for 1.2.1.27
• PubMed Central references for 1.2.1.27
• Google Scholar references for 1.2.1.27
• Sokatch JR, Sanders LE, Marshall VP (1968). "Oxidation of methylmalonate semialdehyde to propionyl coenzyme A in Pseudomonas aeruginosa grown on valine". J. Biol. Chem. 243: 2500–6. PMID 4297649. 
• Rahuel-Clermont S, Branlant G, Aubry A (2004). "Expression, purification, crystallization and preliminary X-ray diffraction data of methylmalonate-semialdehyde dehydrogenase from Bacillus subtilis". Acta. Crystallogr. D. Biol. Crystallogr. 60: 1435–7. doi:10.1107/S0907444904012533. PMID 15272169. 
• Stines-Chaumeil C, Talfournier F, Branlant G (2006). "Mechanistic characterization of the MSDH (methylmalonate semialdehyde dehydrogenase) from Bacillus subtilis". Biochem. J. 395: 107–15. doi:10.1042/BJ20051525. PMID 16332250. 

[edit] External links

The CAS registry number for this enzyme class is 37205-49-5.

• IUBMB entry for 1.2.1.27

• KEGG entry for 1.2.1.27

• BRENDA entry for 1.2.1.27

• NiceZyme view of 1.2.1.27

• EC2PDB: PDB structures for 1.2.1.27

• PRIAM entry for 1.2.1.27

• PUMA2 entry for 1.2.1.27

• IntEnz: Integrated Enzyme entry for 1.2.1.27

• MetaCyc entry for 1.2.1.27

• Atomic-resolution structures of enzymes belonging to this class

[edit] Gene Ontology (GO) codes

• EGO entry for GO code 0004491: methylmalonate-semialdehyde dehydrogenase (acylating)

• AMIGO entry for GO code 0004491: methylmalonate-semialdehyde dehydrogenase (acylating)