Methylenetetrahydrofolate dehydrogenase (NADP+)

From Wikipedia, the free encyclopedia

In enzymology, a methylenetetrahydrofolate dehydrogenase (NADP+) (EC 1.5.1.5) is an enzyme that catalyzes the chemical reaction

5,10-methylenetetrahydrofolate + NADP⁺ 5,10-methenyltetrahydrofolate + NADPH + H⁺

Thus, the two substrates of this enzyme are 5,10-methylenetetrahydrofolate and NADP⁺, whereas its 3 products are 5,10-methenyltetrahydrofolate, NADPH, and H⁺.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-NH group of donors with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is 5,10-methylenetetrahydrofolate:NADP+ oxidoreductase. Other names in common use include N5,N10-methylenetetrahydrofolate dehydrogenase, 5,10-methylenetetrahydrofolate:NADP oxidoreductase, 5,10-methylenetetrahydrofolate dehydrogenase, methylenetetrahydrofolate dehydrogenase, and methylenetetrahydrofolate dehydrogenase (NADP). This enzyme participates in glyoxylate and dicarboxylate metabolism and one carbon pool by folate.

Contents 1 Structural studies 2 References 3 External links 3.1 Gene Ontology (GO) codes

[edit] Structural studies

As of late 2007, 8 structures have been solved for this class of enzymes, with PDB accession codes 1A4I, 1DIA, 1DIB, 1DIG, 1LU9, 1LUA, 2C2X, and 2C2Y.

[edit] References

• IUBMB entry for 1.5.1.5
• BRENDA references for 1.5.1.5 (Recommended.)
• PubMed references for 1.5.1.5
• PubMed Central references for 1.5.1.5
• Google Scholar references for 1.5.1.5
• HATEFI Y, OSBORN MJ, KAY LD, HUENNEKENS FM (1957). "Hydroxymethyl tetrahydrofolic dehydrogenase". J. Biol. Chem. 227: 637–47. PMID 13462986. 
• OSBORN MJ, HUENNEKENS FM (1957). "Participation of anhydroleucovorin in the hydroxymethyl tetrahydrofolic dehydrogenase system". Biochim. Biophys. Acta. 26: 646–7. doi:10.1016/0006-3002(57)90117-8. PMID 13499428. 
• RAMASASTRI BV, BLAKLEY RL (1962). "5,10-Methylenetetrahydrofolic dehydrogenase from bakers' yeast. I Partial purification and some properties". J. Biol. Chem. 237: 1982–8. PMID 14490085. 
• Yeh YC, Greenberg DM (1965). "Purification and properties of N5, N10-Methylenetetra-hydrofolate dehydrogenase of calf thymus". Biochim. Biophys. Acta. 105: 279–91. PMID 4379024. 

[edit] External links

The CAS registry number for this enzyme class is 9029-14-5.

• IUBMB entry for 1.5.1.5

• KEGG entry for 1.5.1.5

• BRENDA entry for 1.5.1.5

• NiceZyme view of 1.5.1.5

• EC2PDB: PDB structures for 1.5.1.5

• PRIAM entry for 1.5.1.5

• PUMA2 entry for 1.5.1.5

• IntEnz: Integrated Enzyme entry for 1.5.1.5

• MetaCyc entry for 1.5.1.5

• Atomic-resolution structures of enzymes belonging to this class

[edit] Gene Ontology (GO) codes

• EGO entry for GO code 0004488: methylenetetrahydrofolate dehydrogenase (NADP+)

• AMIGO entry for GO code 0004488: methylenetetrahydrofolate dehydrogenase (NADP+)