MCM2

From Wikipedia, the free encyclopedia

MCM2 minichromosome maintenance deficient 2, mitotin (S. cerevisiae)

Identifiers

MCM2; BM28; CCNL1; CDCL1; D3S3194; KIAA0030; MGC10606; MITOTIN; cdc19

External IDs

OMIM: 116945 MGI: 105380 HomoloGene: 3325

Gene ontology
Molecular Function:	• nucleotide binding • DNA binding • DNA replication origin binding • protein binding • ATP binding • DNA-dependent ATPase activity • zinc ion binding • metal ion binding
Cellular Component:	• chromatin • nucleus • nuclear origin of replication recognition complex
Biological Process:	• DNA replication • DNA unwinding during replication • DNA replication initiation • nucleosome assembly • transcription • regulation of transcription, DNA-dependent • cell cycle

RNA expression pattern

More reference expression data

Orthologs

Human

Mouse

Refseq

NM_004526 (mRNA)
NP_004517 (protein)

NM_008564 (mRNA)
NP_032590 (protein)

Location

Chr 3: 128.8 - 128.82 Mb

Chr 6: 88.85 - 88.86 Mb

Pubmed search

[1]

[2]

MCM2 minichromosome maintenance deficient 2, mitotin (S. cerevisiae), also known as MCM2, is a human gene.

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7.^[1]

[edit] References

^ Entrez Gene: MCM2 MCM2 minichromosome maintenance deficient 2, mitotin (S. cerevisiae).

[edit] Further reading

Todorov IT, Lavigne J, Sakr F, et al. (1991). "Nuclear matrix protein mitotin messenger RNA is expressed at constant levels during the cell cycle.". Biochem. Biophys. Res. Commun. 177 (1): 395–400. PMID 1710453.
Nomura N, Miyajima N, Sazuka T, et al. (1995). "Prediction of the coding sequences of unidentified human genes. I. The coding sequences of 40 new genes (KIAA0001-KIAA0040) deduced by analysis of randomly sampled cDNA clones from human immature myeloid cell line KG-1.". DNA Res. 1 (1): 27–35. PMID 7584026.
Nomura N, Miyajima N, Sazuka T, et al. (1995). "Prediction of the coding sequences of unidentified human genes. I. The coding sequences of 40 new genes (KIAA0001-KIAA0040) deduced by analysis of randomly sampled cDNA clones from human immature myeloid cell line KG-1 (supplement).". DNA Res. 1 (1): 47–56. PMID 7584028.
Nakatsuru S, Sudo K, Nakamura Y (1995). "Isolation and mapping of a human gene (MCM2) encoding a product homologous to yeast proteins involved in DNA replication.". Cytogenet. Cell Genet. 68 (3-4): 226–30. PMID 7842741.
Todorov IT, Pepperkok R, Philipova RN, et al. (1994). "A human nuclear protein with sequence homology to a family of early S phase proteins is required for entry into S phase and for cell division.". J. Cell. Sci. 107 ( Pt 1): 253–65. PMID 8175912.
Mincheva A, Todorov I, Werner D, et al. (1994). "The human gene for nuclear protein BM28 (CDCL1), a new member of the early S-phase family of proteins, maps to chromosome band 3q21.". Cytogenet. Cell Genet. 65 (4): 276–7. PMID 8258304.
Ishimi Y, Ichinose S, Omori A, et al. (1996). "Binding of human minichromosome maintenance proteins with histone H3.". J. Biol. Chem. 271 (39): 24115–22. PMID 8798650.
Fujita M, Yamada C, Tsurumi T, et al. (1998). "Cell cycle- and chromatin binding state-dependent phosphorylation of human MCM heterohexameric complexes. A role for cdc2 kinase.". J. Biol. Chem. 273 (27): 17095–101. PMID 9642275.
Jiang W, McDonald D, Hope TJ, Hunter T (1999). "Mammalian Cdc7-Dbf4 protein kinase complex is essential for initiation of DNA replication.". EMBO J. 18 (20): 5703–13. doi:10.1093/emboj/18.20.5703. PMID 10523313.
You Z, Komamura Y, Ishimi Y (2000). "Biochemical analysis of the intrinsic Mcm4-Mcm6-mcm7 DNA helicase activity.". Mol. Cell. Biol. 19 (12): 8003–15. PMID 10567526.
Ishimi Y, Komamura-Kohno Y, You Z, et al. (2000). "Inhibition of Mcm4,6,7 helicase activity by phosphorylation with cyclin A/Cdk2.". J. Biol. Chem. 275 (21): 16235–41. doi:10.1074/jbc.M909040199. PMID 10748114.
Masai H, Matsui E, You Z, et al. (2000). "Human Cdc7-related kinase complex. In vitro phosphorylation of MCM by concerted actions of Cdks and Cdc7 and that of a criticial threonine residue of Cdc7 bY Cdks.". J. Biol. Chem. 275 (37): 29042–52. doi:10.1074/jbc.M002713200. PMID 10846177.
Izumi M, Yanagi K, Mizuno T, et al. (2001). "The human homolog of Saccharomyces cerevisiae Mcm10 interacts with replication factors and dissociates from nuclease-resistant nuclear structures in G(2) phase.". Nucleic Acids Res. 28 (23): 4769–77. PMID 11095689.
Burke TW, Cook JG, Asano M, Nevins JR (2001). "Replication factors MCM2 and ORC1 interact with the histone acetyltransferase HBO1.". J. Biol. Chem. 276 (18): 15397–408. doi:10.1074/jbc.M011556200. PMID 11278932.
Tan DF, Huberman JA, Hyland A, et al. (2002). "MCM2--a promising marker for premalignant lesions of the lung: a cohort study.". BMC Cancer 1: 6. PMID 11472637.
Ishimi Y, Komamura-Kohno Y, Arai K, Masai H (2001). "Biochemical activities associated with mouse Mcm2 protein.". J. Biol. Chem. 276 (46): 42744–52. doi:10.1074/jbc.M106861200. PMID 11568184.
Wohlschlegel JA, Dhar SK, Prokhorova TA, et al. (2002). "Xenopus Mcm10 binds to origins of DNA replication after Mcm2-7 and stimulates origin binding of Cdc45.". Mol. Cell 9 (2): 233–40. PMID 11864598.
Kodani I, Shomori K, Osaki M, et al. (2002). "Expression of minichromosome maintenance 2 (MCM2), Ki-67, and cell-cycle-related molecules, and apoptosis in the normal-dysplasia-carcinoma sequence of the oral mucosa.". Pathobiology 69 (3): 150–8. PMID 11872961.
Edwards MC, Tutter AV, Cvetic C, et al. (2002). "MCM2-7 complexes bind chromatin in a distributed pattern surrounding the origin recognition complex in Xenopus egg extracts.". J. Biol. Chem. 277 (36): 33049–57. doi:10.1074/jbc.M204438200. PMID 12087101.
Osaki M, Osaki M, Yamashita H, et al. (2003). "Expression of minichromosome maintenance-2 in human malignant fibrous histiocytomas: Correlations with Ki-67 and P53 expression, and apoptosis.". Int. J. Mol. Med. 10 (2): 161–8. PMID 12119552.