Major basic protein

From Wikipedia, the free encyclopedia

Proteoglycan 2, bone marrow (natural killer cell activator, eosinophil granule major basic protein)

PDB rendering based on 1h8u.

Available structures: 1h8u, 2brs

Identifiers

Symbol(s)

PRG2; MBP; BMPG; MGC14537

External IDs

OMIM: 605601 MGI: 103294 HomoloGene: 2044

Gene ontology
Molecular Function:	• sugar binding • heparin binding
Cellular Component:	• extracellular region • cytoplasm
Biological Process:	• inflammatory response • immune response • defense response to bacterium

RNA expression pattern

More reference expression data

Orthologs

Human

Mouse

Refseq

NM_002728 (mRNA)
NP_002719 (protein)

NM_008920 (mRNA)
NP_032946 (protein)

Location

Chr 11: 56.91 - 56.91 Mb

Chr 2: 84.78 - 84.78 Mb

Pubmed search

[1]

[2]

Proteoglycan 2, bone marrow (natural killer cell activator, eosinophil granule major basic protein), also known as PRG2, is a human gene.^[1]

The protein encoded by this gene is the predominant constituent of the crystalline core of the eosinophil granule. High levels of the proform of this protein are also present in placenta and pregnancy serum, where it exists as a complex with several other proteins including pregnancy-associated plasma protein A (PAPPA), angiotensinogen (AGT), and C3dg. This protein may be involved in antiparasitic defense mechanisms as a cytotoxin and helminthotoxin, and in immune hypersensitivity reactions. It is directly implicated in epithelial cell damage, exfoliation, and bronchospasm in allergic diseases.^[1]

A 117-residue protein that predominates in eosinophil granules. It is a potent enzyme against helminths and is toxic towards bacteria and mammalian cells in vitro. The eosinophil major basic protein also causes the release of histamine from mast cells and basophils, activates neutrophils and alveolar macrophages, and is directly implicated in epithelial cell damage, exfoliation and bronchospasm in asthma.

Structurally the major basic protein (MBP) is similar to lectins (sugar-binding proteins), and has a fold similar to that seen in C-type lectins. However, unlike other C-type lectins (those that bind various carbohydrates in the presence of calcium), MBP does not bind either calcium or any of the other carbohydrates that this family recognize.

Instead, MBP recognises heparan sulfate proteoglycans. The structure of MBP is available from the Protein Data Bank, accession number 1h8u^[2] and 2brs^[3]

[edit] External links

MeSH Eosinophil+Major+Basic+Protein

[edit] References

^ ^a ^b Entrez Gene: PRG2 proteoglycan 2, bone marrow (natural killer cell activator, eosinophil granule major basic protein).
^ Crystal structure of the eosinophil major basic protein at 1.8 A. An atypical lectin with a paradigm shift in specificity. J Biol Chem. 276:26197-203, 2001
^ Eosinophil-granule major basic protein, a C-type lectin, binds heparin. Biochemistry. 44:14152-8, 2005.

[edit] Further reading

Vanhaesebroeck B, Alessi DR (2000). "The PI3K-PDK1 connection: more than just a road to PKB.". Biochem. J. 346 Pt 3: 561–76. PMID 10698680.
Yoshimatsu K, Ohya Y, Shikata Y, et al. (1992). "Purification and cDNA cloning of a novel factor produced by a human T-cell hybridoma: sequence homology with animal lectins.". Mol. Immunol. 29 (4): 537–46. PMID 1565101.
Barker RL, Loegering DA, Arakawa KC, et al. (1990). "Cloning and sequence analysis of the human gene encoding eosinophil major basic protein.". Gene 86 (2): 285–9. PMID 2323577.
Gabay JE, Scott RW, Campanelli D, et al. (1989). "Antibiotic proteins of human polymorphonuclear leukocytes.". Proc. Natl. Acad. Sci. U.S.A. 86 (14): 5610–4. PMID 2501794.
Wasmoen TL, McKean DJ, Benirschke K, et al. (1990). "Evidence of eosinophil granule major basic protein in human placenta.". J. Exp. Med. 170 (6): 2051–63. PMID 2584934.
Barker RL, Gleich GJ, Pease LR (1988). "Acidic precursor revealed in human eosinophil granule major basic protein cDNA.". J. Exp. Med. 168 (4): 1493–8. PMID 3171483.
McGrogan M, Simonsen C, Scott R, et al. (1989). "Isolation of a complementary DNA clone encoding a precursor to human eosinophil major basic protein.". J. Exp. Med. 168 (6): 2295–308. PMID 3199069.
Wasmoen TL, Bell MP, Loegering DA, et al. (1988). "Biochemical and amino acid sequence analysis of human eosinophil granule major basic protein.". J. Biol. Chem. 263 (25): 12559–63. PMID 3410852.
Weller PF, Ackerman SJ, Smith JA (1988). "Eosinophil granule cationic proteins: major basic protein is distinct from the smaller subunit of eosinophil peroxidase.". J. Leukoc. Biol. 43 (1): 1–4. PMID 3422083.
Kristensen T, Oxvig C, Sand O, et al. (1994). "Amino acid sequence of human pregnancy-associated plasma protein-A derived from cloned cDNA.". Biochemistry 33 (6): 1592–8. PMID 7508748.
Oxvig C, Haaning J, Højrup P, Sottrup-Jensen L (1994). "Location and nature of carbohydrate groups in proform of human major basic protein isolated from pregnancy serum.". Biochem. Mol. Biol. Int. 33 (2): 329–36. PMID 7524900.
Bonno M, Oxvig C, Kephart GM, et al. (1994). "Localization of pregnancy-associated plasma protein-A and colocalization of pregnancy-associated plasma protein-A messenger ribonucleic acid and eosinophil granule major basic protein messenger ribonucleic acid in placenta.". Lab. Invest. 71 (4): 560–6. PMID 7526035.
Li MS, Sun L, Satoh T, et al. (1995). "Human eosinophil major basic protein, a mediator of allergic inflammation, is expressed by alternative splicing from two promoters.". Biochem. J. 305 ( Pt 3): 921–7. PMID 7531438.
Oxvig C, Haaning J, Kristensen L, et al. (1995). "Identification of angiotensinogen and complement C3dg as novel proteins binding the proform of eosinophil major basic protein in human pregnancy serum and plasma.". J. Biol. Chem. 270 (23): 13645–51. PMID 7539791.
Oxvig C, Sand O, Kristensen T, et al. (1993). "Circulating human pregnancy-associated plasma protein-A is disulfide-bridged to the proform of eosinophil major basic protein.". J. Biol. Chem. 268 (17): 12243–6. PMID 7685339.
Levi-Schaffer F, Lacy P, Severs NJ, et al. (1995). "Association of granulocyte-macrophage colony-stimulating factor with the crystalloid granules of human eosinophils.". Blood 85 (9): 2579–86. PMID 7727786.
Oxvig C, Gleich GJ, Sottrup-Jensen L (1994). "Localization of disulfide bridges and free sulfhydryl groups in human eosinophil granule major basic protein.". FEBS Lett. 341 (2-3): 213–7. PMID 8137941.
Shikata Y, Hayashi Y, Yoshimatsu K, et al. (1993). "Pro-major basic protein has three types of sugar chains at the pro-portion.". Biochim. Biophys. Acta 1163 (3): 243–9. PMID 8507662.
Nittoh T, Watanabe M, Okayama H, et al. (1996). "Cloning of cDNA for rat eosinophil major basic protein.". Biochim. Biophys. Acta 1264 (3): 261–4. PMID 8547309.
Delcommenne M, Tan C, Gray V, et al. (1998). "Phosphoinositide-3-OH kinase-dependent regulation of glycogen synthase kinase 3 and protein kinase B/AKT by the integrin-linked kinase.". Proc. Natl. Acad. Sci. U.S.A. 95 (19): 11211–6. PMID 9736715.