Lysine-tRNA ligase

From Wikipedia, the free encyclopedia

In enzymology, a lysine-tRNA ligase (EC 6.1.1.6) is an enzyme that catalyzes the chemical reaction

ATP + L-lysine + tRNALys AMP + diphosphate + L-lysyl-tRNALys

The 3 substrates of this enzyme are ATP, L-lysine, and tRNA(Lys), whereas its 3 products are AMP, diphosphate, and L-lysyl-tRNA(Lys).

This enzyme belongs to the family of ligases, specifically those forming carbon-oxygen bonds in aminoacyl-tRNA and related compounds. The systematic name of this enzyme class is L-lysine:tRNALys ligase (AMP-forming). Other names in common use include lysyl-tRNA synthetase, lysyl-transfer ribonucleate synthetase, lysyl-transfer RNA synthetase, L-lysine-transfer RNA ligase, lysine-tRNA synthetase, and lysine translase. This enzyme participates in 3 metabolic pathways: lysine biosynthesis, aminoacyl-trna biosynthesis, and amyotrophic lateral sclerosis (als).

Contents 1 Structural studies 2 References 3 External links 3.1 Gene Ontology (GO) codes

[edit] Structural studies

As of late 2007, 10 structures have been solved for this class of enzymes, with PDB accession codes 1BBU, 1BBW, 1E1O, 1E1T, 1E22, 1E24, 1IRX, 1KRS, 1KRT, and 1LYL.

[edit] References

• IUBMB entry for 6.1.1.6
• BRENDA references for 6.1.1.6 (Recommended.)
• PubMed references for 6.1.1.6
• PubMed Central references for 6.1.1.6
• Google Scholar references for 6.1.1.6
• ALLEN EH, GLASSMAN E, SCHWEET RS (1960). "Incorporation of amino acids into ribonucleic acid. I. The role of activating enzymes". J. Biol. Chem. 235: 1061–7. PMID 13792726. 
• Chlumecka V, Von Tigerstrom M, D'Obrenan P, Smith CJ (1969). "Purification and properties of lysyl transfer ribonucleic acid synthetase from bakers' yeast". J. Biol. Chem. 244: 5481–8. PMID 4310598. 
• Lagerkvist U, Rymo L, Lindqvist O, Andersson E (1972). "Some properties of crystals of lysine transfer ribonucleic acid ligase from yeast". J. Biol. Chem. 247: 3897–9. PMID 4555953. 
• Stern R, Mehler AH (1965). "Lysyl-sRNA synthetase from Escherichia coli". Biochem. Z. 342: 400–9. PMID 4284804. 

[edit] External links

The CAS registry number for this enzyme class is 9031-26-9.

• IUBMB entry for 6.1.1.6

• KEGG entry for 6.1.1.6

• BRENDA entry for 6.1.1.6

• NiceZyme view of 6.1.1.6

• EC2PDB: PDB structures for 6.1.1.6

• PRIAM entry for 6.1.1.6

• PUMA2 entry for 6.1.1.6

• IntEnz: Integrated Enzyme entry for 6.1.1.6

• MetaCyc entry for 6.1.1.6

• Atomic-resolution structures of enzymes belonging to this class

[edit] Gene Ontology (GO) codes

• EGO entry for GO code 0004824: lysine-tRNA ligase

• AMIGO entry for GO code 0004824: lysine-tRNA ligase