Lymphocyte cytosolic protein 2

From Wikipedia, the free encyclopedia

Lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of 76kDa)

Identifiers

LCP2; SLP-76; SLP76

External IDs

OMIM: 601603 MGI: 1321402 HomoloGene: 4065

Gene ontology
Molecular Function:	• protein binding
Biological Process:	• immune response • transmembrane receptor protein tyrosine kinase signaling pathway • intracellular signaling cascade • mast cell activation • cytokine secretion

RNA expression pattern

More reference expression data

Orthologs

Human

Mouse

Refseq

NM_005565 (mRNA)
NP_005556 (protein)

NM_010696 (mRNA)
NP_034826 (protein)

Location

Chr 5: 169.61 - 169.66 Mb

Chr 11: 33.95 - 33.99 Mb

Pubmed search

[1]

[2]

Lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of 76kDa), also known as LCP2 or SLP-76, is a gene which encodes a signal transducing adaptor protein.^[1]

SLP-76 was originally identified as a substrate of the ZAP-70 protein tyrosine kinase following T cell receptor (TCR) ligation in the leukemic T cell line Jurkat. The SLP-76 locus has been localized to human chromosome 5q33 and the gene structure has been partially characterized in mice. The human and murine cDNAs both encode 533 amino acid proteins that are 72% identical and comprised of three modular domains. The NH2-terminus contains an acidic region that includes a PEST domain and several tyrosine residues which are phosphorylated following TCR ligation. SLP-76 also contains a central proline-rich domain and a COOH-terminal SH2 domain. A number of additional proteins have been identified that associate with SLP-76 both constitutively and inducibly following receptor ligation, supporting the notion that SLP-76 functions as an adaptor or scaffold protein. Studies using SLP-76 deficient T cell lines or mice have provided strong evidence that SLP-76 plays a positive role in promoting T cell development and activation as well as mast cell and platelet function.^[1]

1 References
2 Further reading
3 See also
4 External links

[edit] References

^ ^a ^b Entrez Gene: LCP2 lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of 76kDa).

[edit] Further reading

Jackman JK, Motto DG, Sun Q, et al. (1995). "Molecular cloning of SLP-76, a 76-kDa tyrosine phosphoprotein associated with Grb2 in T cells.". J. Biol. Chem. 270 (13): 7029–32. PMID 7706237.
Sunden SL, Carr LL, Clements JL, et al. (1996). "Polymorphism in and localization of the gene LCP2 (SLP-76) to chromosome 5q33.1-qter.". Genomics 35 (1): 269–70. doi:10.1006/geno.1996.0354. PMID 8661136.
Motto DG, Ross SE, Wu J, et al. (1996). "Implication of the GRB2-associated phosphoprotein SLP-76 in T cell receptor-mediated interleukin 2 production.". J. Exp. Med. 183 (4): 1937–43. PMID 8666952.
Wardenburg JB, Fu C, Jackman JK, et al. (1996). "Phosphorylation of SLP-76 by the ZAP-70 protein-tyrosine kinase is required for T-cell receptor function.". J. Biol. Chem. 271 (33): 19641–4. PMID 8702662.
Onodera H, Motto DG, Koretzky GA, Rothstein DM (1996). "Differential regulation of activation-induced tyrosine phosphorylation and recruitment of SLP-76 to Vav by distinct isoforms of the CD45 protein-tyrosine phosphatase.". J. Biol. Chem. 271 (36): 22225–30. PMID 8703037.
Mizuno K, Katagiri T, Hasegawa K, et al. (1996). "Hematopoietic cell phosphatase, SHP-1, is constitutively associated with the SH2 domain-containing leukocyte protein, SLP-76, in B cells.". J. Exp. Med. 184 (2): 457–63. PMID 8760799.
Raab M, da Silva AJ, Findell PR, Rudd CE (1997). "Regulation of Vav-SLP-76 binding by ZAP-70 and its relevance to TCR zeta/CD3 induction of interleukin-2.". Immunity 6 (2): 155–64. PMID 9047237.
Musci MA, Hendricks-Taylor LR, Motto DG, et al. (1997). "Molecular cloning of SLAP-130, an SLP-76-associated substrate of the T cell antigen receptor-stimulated protein tyrosine kinases.". J. Biol. Chem. 272 (18): 11674–7. PMID 9115214.
da Silva AJ, Li Z, de Vera C, et al. (1997). "Cloning of a novel T-cell protein FYB that binds FYN and SH2-domain-containing leukocyte protein 76 and modulates interleukin 2 production.". Proc. Natl. Acad. Sci. U.S.A. 94 (14): 7493–8. PMID 9207119.
Fu C, Chan AC (1997). "Identification of two tyrosine phosphoproteins, pp70 and pp68, which interact with phospholipase Cgamma, Grb2, and Vav after B cell antigen receptor activation.". J. Biol. Chem. 272 (43): 27362–8. PMID 9341187.
Zhang W, Sloan-Lancaster J, Kitchen J, et al. (1998). "LAT: the ZAP-70 tyrosine kinase substrate that links T cell receptor to cellular activation.". Cell 92 (1): 83–92. PMID 9489702.
Fu C, Turck CW, Kurosaki T, Chan AC (1998). "BLNK: a central linker protein in B cell activation.". Immunity 9 (1): 93–103. PMID 9697839.
Chu J, Liu Y, Koretzky GA, Durden DL (1998). "SLP-76-Cbl-Grb2-Shc interactions in FcgammaRI signaling.". Blood 92 (5): 1697–706. PMID 9716598.
Binstadt BA, Billadeau DD, Jevremović D, et al. (1998). "SLP-76 is a direct substrate of SHP-1 recruited to killer cell inhibitory receptors.". J. Biol. Chem. 273 (42): 27518–23. PMID 9765283.
Liu SK, Fang N, Koretzky GA, McGlade CJ (1999). "The hematopoietic-specific adaptor protein gads functions in T-cell signaling via interactions with the SLP-76 and LAT adaptors.". Curr. Biol. 9 (2): 67–75. PMID 10021361.
Gross BS, Lee JR, Clements JL, et al. (1999). "Tyrosine phosphorylation of SLP-76 is downstream of Syk following stimulation of the collagen receptor in platelets.". J. Biol. Chem. 274 (9): 5963–71. PMID 10026222.
Erdreich-Epstein A, Liu M, Kant AM, et al. (1999). "Cbl functions downstream of Src kinases in Fc gamma RI signaling in primary human macrophages.". J. Leukoc. Biol. 65 (4): 523–34. PMID 10204582.
Asada H, Ishii N, Sasaki Y, et al. (1999). "Grf40, A novel Grb2 family member, is involved in T cell signaling through interaction with SLP-76 and LAT.". J. Exp. Med. 189 (9): 1383–90. PMID 10224278.
Wunderlich L, Faragó A, Downward J, Buday L (1999). "Association of Nck with tyrosine-phosphorylated SLP-76 in activated T lymphocytes.". Eur. J. Immunol. 29 (4): 1068–75. PMID 10229072.
Gross BS, Melford SK, Watson SP (1999). "Evidence that phospholipase C-gamma2 interacts with SLP-76, Syk, Lyn, LAT and the Fc receptor gamma-chain after stimulation of the collagen receptor glycoprotein VI in human platelets.". Eur. J. Biochem. 263 (3): 612–23. PMID 10469124.