Lowry protein assay

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The Lowry protein assay, or Lowry Method, is a biochemical test to measure the amount of protein in a sample.

Copper(II) ion in alkaline solution reacts with protein to form complexes, which react with the Folin-phenol reagent, a mixture of phosphotungstic acid and phosphomolybdic acid in phenol. The product becomes reduced to molybdenum/tungsten blue and can be detected colorimetrically by absorbance at 750 nm. The higher the concentration of protein, the darker the solution.

Presence of strong acids or ammonium sulfate can interfere with the assay.

[edit] External links

• Lowry and Modified Lowry Protein Assays

v • d • e Proteins: key methods of study Experimental Protein purification - Green fluorescent protein - Western blot - Protein immunostaining - Protein sequencing - Gel electrophoresis/Protein electrophoresis - Protein immunoprecipitation - Peptide mass fingerprinting Bioinformatics Protein structure prediction - Protein-protein docking - Protein structural alignment - Protein ontology - Protein-protein interaction prediction Assay Enzyme assay - Protein assay - Secretion assay