Lowenstein-Jensen medium

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The Lowenstein-Jensen medium, popularly known as LJ medium, is a growth medium specially used for culture of Mycobacterium, notably Mycobacterium tuberculosis.

[edit] Composition

The usual composition^[1] as applicable to Mycobacterium tuberculosis is:

• Malachite green
• Glycerol
• Asparagine
• Potato flour
• Coagulated eggs
• Mineral salt solution
  • Potassium dihydrogen phosphate
  • Magnesium sulfate
  • Sodium citrate

For cultivation of M.bovis, glycerol is omitted and sodium pyruvate is added.

[edit] References

1. ^ Composition of LJ medium