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Bernheim Arboretum and Research Forest is a 14,000 acre (57 km²) arboretum, forest, and nature preserve located in Clermont, Kentucky (south of Louisville, Kentucky, United States). It was founded in 1929 by Isaac Wolfe Bernheim, a German immigrant and successful brewer whose whiskey distillery business established the I.W. Harper brand. He purchased the land in 1928 at $1 an acre because most of it had been stripped for mining iron ore.

The property includes a 240-acre (0.97 km²) arboretum containing over 1,900 labeled species and cultivars of trees, shrubs, and other plants. The arboretum includes over 185 cultivars of American holly species. Other major collections include maples, crab apples, conifers (including dwarf conifers), oaks, buckeyes, ginkgoes, ornamental pears, and dogwoods. Specific attractions within the arboretum include the sun and shade trail, quiet garden, and garden pavilion.