Lithocholate 6beta-hydroxylase

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In enzymology, a lithocholate 6beta-hydroxylase (EC 1.14.13.94) is an enzyme that catalyzes the chemical reaction

lithocholate + NADPH + H⁺ + O₂ $\rightleftharpoons$ 6beta-hydroxylithocholate + NADP⁺ + H₂O

The 4 substrates of this enzyme are lithocholate, NADPH, H⁺, and O₂, whereas its 3 products are 6beta-hydroxylithocholate, NADP⁺, and H₂O.

This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with NADH or NADPH as one donor, and incorporation of one atom o oxygen into the other donor. The systematic name of this enzyme class is lithocholate,NADPH:oxygen oxidoreductase (6beta-hydroxylating). Other names in common use include lithocholate 6beta-monooxygenase, CYP3A10, 6beta-hydroxylase, and cytochrome P450 3A10/lithocholic acid 6beta-hydroxylase.

[edit] References

IUBMB entry for 1.14.13.94
BRENDA references for 1.14.13.94 (Recommended.)
PubMed references for 1.14.13.94
PubMed Central references for 1.14.13.94
Google Scholar references for 1.14.13.94
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Subramanian A, Wang J, Gil G (1998). "STAT 5 and NF-Y are involved in expression and growth hormone-mediated sexually dimorphic regulation of cytochrome P450 3A10/lithocholic acid 6beta-hydroxylase". Nucleic. Acids. Res. 26: 2173–8. doi:10.1093/nar/26.9.2173. PMID 9547277.
Russell DW (2003). "The enzymes, regulation, and genetics of bile acid synthesis". Annu. Rev. Biochem. 72: 137–74. doi:10.1146/annurev.biochem.72.121801.161712. PMID 12543708.