List of standard amino acids

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This list of standard proteinogenic amino acids details the chemical structures and properties of the twenty standard amino acids used in proteins by living cells.

Contents 1 Structures 2 Chemical properties 2.1 General chemical properties 2.2 Side chain properties 2.3 Gene expression and biochemistry 2.4 Remarks 3 References

[edit] Structures

Structures and symbols of the 20 amino acids which are directly encoded for protein synthesis by the standard genetic code

L-Alanine (Ala / A)	L-Arginine (Arg / R)	L-Asparagine (Asn / N)	L-Aspartic acid (Asp / D)
L-Cysteine (Cys / C)	L-Glutamic acid (Glu / E)	L-Glutamine (Gln / Q)	Glycine (Gly / G)
L-Histidine (His / H)	L-Isoleucine (Ile / I)	L-Leucine (Leu / L)	L-Lysine (Lys / K)
L-Methionine (Met / M)	L-Phenylalanine (Phe / F)	L-Proline (Pro / P)	L-Serine (Ser / S)
L-Threonine (Thr / T)	L-Tryptophan (Trp / W)	L-Tyrosine (Tyr / Y)	L-Valine (Val / V)

[edit] Chemical properties

Following is a table listing the one-letter symbols, the three-letter symbols, and the chemical properties of the side chains of the standard amino acids. The masses listed are based on weighted averages of the elemental isotopes at their natural abundances. Note that forming a peptide bond results in elimination of a molecule of water, so the mass of an amino acid unit within a protein chain is reduced by 18.01524 Da. The one-letter symbol for an undetermined amino acid is X. The three-letter symbol Asx or one-letter symbol B means the amino acid is either asparagine or aspartic acid; Glx or Z means either glutamic acid or glutamine; and Xle or J means either leucine or isoleucine. IUPAC/IUBMB now also recommends that Sec or U refers to selenocysteine, and Pyl or O refers to pyrrolysine.

[edit] General chemical properties

Amino Acid	Short	Abbrev.	Avg. Mass (Da)	pI	pK1 (α-COOH)	pK2 (α-+NH3)
Alanine	A	Ala	89.09404	6.01	2.35	9.87
Cysteine	C	Cys	121.15404	5.05	1.92	10.70
Aspartic acid	D	Asp	133.10384	2.85	1.99	9.90
Glutamic acid	E	Glu	147.13074	3.15	2.10	9.47
Phenylalanine	F	Phe	165.19184	5.49	2.20	9.31
Glycine	G	Gly	75.06714	6.06	2.35	9.78
Histidine	H	His	155.15634	7.60	1.80	9.33
Isoleucine	I	Ile	131.17464	6.05	2.32	9.76
Lysine	K	Lys	146.18934	9.60	2.16	9.06
Leucine	L	Leu	131.17464	6.01	2.33	9.74
Methionine	M	Met	149.20784	5.74	2.13	9.28
Asparagine	N	Asn	132.11904	5.41	2.14	8.72
Pyrrolysine	O	Pyl
Proline	P	Pro	115.13194	6.30	1.95	10.64
Glutamine	Q	Gln	146.14594	5.65	2.17	9.13
Arginine	R	Arg	174.20274	10.76	1.82	8.99
Serine	S	Ser	105.09344	5.68	2.19	9.21
Threonine	T	Thr	119.12034	5.60	2.09	9.10
Selenocysteine	U	Sec	169.06
Valine	V	Val	117.14784	6.00	2.39	9.74
Tryptophan	W	Trp	204.22844	5.89	2.46	9.41
Tyrosine	Y	Tyr	181.19124	5.64	2.20	9.21

[edit] Side chain properties

Amino Acid	Short	Abbrev.	Side chain	Hydro- phobic	pKa	Polar	pH	Small	Tiny	Aromatic or Aliphatic	van der Waals volume
Alanine	A	Ala	-CH3	X	-	-	-	X	X	-	67
Cysteine	C	Cys	-CH2SH	-	8.18	-	acidic	X	-	-	86
Aspartic acid	D	Asp	-CH2COOH	-	3.90	X	acidic	X	-	-	91
Glutamic acid	E	Glu	-CH2CH2COOH	-	4.07	X	acidic	-	-	-	109
Phenylalanine	F	Phe	-CH2C6H5	X	-	-	-	-	-	Aromatic	135
Glycine	G	Gly	-H	-	-	-	-	X	X	-	48
Histidine	H	His	-CH2-C3H3N2	-	6.04	X	weak basic	-	-	Aromatic	118
Isoleucine	I	Ile	-CH(CH3)CH2CH3	X	-	-	-	-	-	Aliphatic	124
Lysine	K	Lys	-(CH2)4NH2	-	10.54	X	basic	-	-	-	135
Leucine	L	Leu	-CH2CH(CH3)2	X	-	-	-	-	-	Aliphatic	124
Methionine	M	Met	-CH2CH2SCH3	X	-	-	-	-	-	-	124
Asparagine	N	Asn	-CH2CONH2	-	-	X	-	X	-	-	96
Pyrrolysine	O	Pyl
Proline	P	Pro	-CH2CH2CH2-	X	-	-	-	X	-	-	90
Glutamine	Q	Gln	-CH2CH2CONH2	-	-	X	-	-	-	-	114
Arginine	R	Arg	-(CH2)3NH-C(NH)NH2	-	12.48	X	strongly basic	-	-	-	148
Serine	S	Ser	-CH2OH	-	-	X	-	X	X	-	73
Threonine	T	Thr	-CH(OH)CH3	-	-	X	weak acidic	X	-	-	93
Selenocysteine	U	Sec	-CH2SeH	X	5.73	-	-	X	-	-
Valine	V	Val	-CH(CH3)2	X	-	-	-	X	-	Aliphatic	105
Tryptophan	W	Trp	-CH2C8H6N	X	-	-	-	-	-	Aromatic	163
Tyrosine	Y	Tyr	-CH2-C6H4OH	-	10.46	X	-	-	-	Aromatic	141

Note: The pKa values of amino acids are typically slightly different when the amino acid is inside a protein. Protein pKa calculations are sometimes used to calculate the change in the pKa value of an amino acid in this situation.

[edit] Gene expression and biochemistry

Amino Acid	Short	Abbrev.	Codon(s)	Occurrence in proteins (%)	Essential‡ in humans
Alanine	A	Ala	GCU, GCC, GCA, GCG	7.8	-
Cysteine	C	Cys	UGU, UGC	1.9	Conditionally
Aspartic acid	D	Asp	GAU, GAC	5.3	-
Glutamic acid	E	Glu	GAA, GAG	6.3	Conditionally
Phenylalanine	F	Phe	UUU, UUC	3.9	Yes
Glycine	G	Gly	GGU, GGC, GGA, GGG	7.2	Conditionally
Histidine	H	His	CAU, CAC	2.3	Yes
Isoleucine	I	Ile	AUU, AUC, AUA	5.3	Yes
Lysine	K	Lys	AAA, AAG	5.9	Yes
Leucine	L	Leu	UUA, UUG, CUU, CUC, CUA, CUG	9.1	Yes
Methionine	M	Met	AUG	2.3	Yes
Asparagine	N	Asn	AAU, AAC	4.3	-
Pyrrolysine	O	Pyl
Proline	P	Pro	CCU, CCC, CCA, CCG	5.2	-
Glutamine	Q	Gln	CAA, CAG	4.2	-
Arginine	R	Arg	CGU, CGC, CGA, CGG, AGA, AGG	5.1	Conditionally
Serine	S	Ser	UCU, UCC, UCA, UCG, AGU, AGC	6.8	-
Threonine	T	Thr	ACU, ACC, ACA, ACG	5.9	Yes
Selenocysteine	U	Sec	UGA*		-
Valine	V	Val	GUU, GUC, GUA, GUG	6.6	Yes
Tryptophan	W	Trp	UGG	1.4	Yes
Tyrosine	Y	Tyr	UAU, UAC	3.2	Conditionally
Stop codon†	-	Term	UAA, UAG, UGA	-	-

* UGA is normally a stop codon, but encodes selenocysteine if a SECIS element is present.
† The stop codon is not an amino acid, but is included for completeness.
‡ An essential amino acid cannot be synthesized in humans and must, therefore, be supplied in the diet. Conditionally essential amino acids are not normally required in the diet, but must be supplied exogenously to specific populations that do not synthesize it in adequate amounts.

[edit] Remarks

Amino Acid	Abbrev.		Remarks
Alanine	A	Ala	Very abundant, very versatile. More stiff than glycine, but small enough to pose only small steric limits for the protein conformation. It behaves fairly neutrally, can be located in both hydrophilic regions on the protein outside and the hydrophobic areas inside.
Cysteine	C	Cys	The sulfur atom binds readily to heavy metal ions. Under oxidizing conditions, two cysteines can join together in a disulfide bond to form the amino acid cystine. When cystines are part of a protein, insulin for example, this stabilises tertiary structure and makes the protein more resistant to denaturation; disulfide bridges are therefore common in proteins that have to function in harsh environments including digestive enzymes (e.g., pepsin and chymotrypsin) and structural proteins (e.g., keratin). Disulfides are also found in peptides too small to hold a stable shape on their own (eg. insulin).
Aspartic acid	D	Asp	Behaves similarly to glutamic acid. Carries a hydrophilic acidic group with strong negative charge. Usually is located on the outer surface of the protein, making it water-soluble. Binds to positively-charged molecules and ions, often used in enzymes to fix the metal ion. When located inside of the protein, aspartate and glutamate are usually paired with arginine and lysine.
Glutamic acid	E	Glu	Behaves similar to aspartic acid. Has longer, slightly more flexible side chain.
Phenylalanine	F	Phe	Essential for humans. Phenylalanine, tyrosine, and tryptophan contain large rigid aromatic group on the side chain. These are the biggest amino acids. Like isoleucine, leucine and valine, these are hydrophobic and tend to orient towards the interior of the folded protein molecule.
Glycine	G	Gly	Because of the two hydrogen atoms at the α carbon, glycine is not optically active. It is the smallest amino acid, rotates easily, adds flexibility to the protein chain. It is able to fit into the tightest spaces, e.g., the triple helix of collagen. As too much flexibility is usually not desired, as a structural component it is less common than alanine.
Histidine	H	His	In even slightly acidic conditions protonation of the nitrogen occurs, changing the properties of histidine and the polypeptide as a whole. It is used by many proteins as a regulatory mechanism, changing the conformation and behavior of the polypeptide in acidic regions such as the late endosome or lysosome, enforcing conformation change in enzymes. However only a few histidines are needed for this, so it is comparatively scarce.
Isoleucine	I	Ile	Essential for humans. Isoleucine, leucine and valine have large aliphatic hydrophobic side chains. Their molecules are rigid, and their mutual hydrophobic interactions are important for the correct folding of proteins, as these chains tend to be located inside of the protein molecule.
Lysine	K	Lys	Essential for humans. Behaves similarly to arginine. Contains a long flexible side-chain with a positively-charged end. The flexibility of the chain makes lysine and arginine suitable for binding to molecules with many negative charges on their surfaces. E.g., DNA-binding proteins have their active regions rich with arginine and lysine. The strong charge makes these two amino acids prone to be located on the outer hydrophilic surfaces of the proteins; when they are found inside, they are usually paired with a corresponding negatively-charged amino acid, e.g., aspartate or glutamate.
Leucine	L	Leu	Essential for humans. Behaves similar to isoleucine and valine. See isoleucine.
Methionine	M	Met	Essential for humans. Always the first amino acid to be incorporated into a protein; sometimes removed after translation. Like cysteine, contains sulfur, but with a methyl group instead of hydrogen. This methyl group can be activated, and is used in many reactions where a new carbon atom is being added to another molecule.
Asparagine	N	Asn	Similar to aspartic acid. Asn contains an amide group where Asp has a carboxyl.
Proline	P	Pro	Contains an unusual ring to the N-end amine group, which forces the CO-NH amide sequence into a fixed conformation. Can disrupt protein folding structures like α helix or β sheet, forcing the desired kink in the protein chain. Common in collagen, where it often undergoes a posttranslational modification to hydroxyproline. Uncommon elsewhere.
Glutamine	Q	Gln	Similar to glutamic acid. Gln contains an amide group where Glu has a carboxyl. Used in proteins and as a storage for ammonia.
Arginine	R	Arg	Functionally similar to lysine.
Serine	S	Ser	Serine and threonine have a short group ended with a hydroxyl group. Its hydrogen is easy to remove, so serine and threonine often act as hydrogen donors in enzymes. Both are very hydrophilic, therefore the outer regions of soluble proteins tend to be rich with them.
Threonine	T	Thr	Essential for humans. Behaves similarly to serine.
Valine	V	Val	Essential for humans. Behaves similarly to isoleucine and leucine. See isoleucine.
Tryptophan	W	Trp	Essential for humans. Behaves similarly to phenylalanine and tyrosine (see phenylalanine). Precursor of serotonin.
Tyrosine	Y	Tyr	Behaves similarly to phenylalanine and tryptophan (see phenylalanine). Precursor of melanin, epinephrine, and thyroid hormones.

[edit] References

• Nelson, David L.; Cox, Michael M. (2000). Lehninger Principles of Biochemistry, 3rd ed, Worth Publishers. ISBN 1-57259-153-6. 
• Kyte, J.; Doolittle, R. F. (1982). "A simple method for displaying the hydropathic character of a protein". J. Mol. Biol. 157 (1): 105–132. doi:10.1016/0022-2836(82)90515-0. PMID 7108955.