Lipoyl synthase

From Wikipedia, the free encyclopedia

In enzymology, a lipoyl synthase (EC 2.8.1.8) is an enzyme that catalyzes the chemical reaction

protein N₆-(octanoyl)lysine + 2 sulfur + 2 S-adenosyl-L-methionine $\rightleftharpoons$ protein N₆-(lipoyl)lysine + 2 L-methionine + 2 5'-deoxyadenosine

The 3 substrates of this enzyme are protein N6-(octanoyl)lysine, sulfur, and S-adenosyl-L-methionine, whereas its 3 products are protein N6-(lipoyl)lysine, L-methionine, and 5'-deoxyadenosine.

This enzyme belongs to the family of transferases, specifically the sulfurtransferases, which transfer sulfur-containing groups. The systematic name of this enzyme class is protein N6-(octanoyl)lysine:sulfur sulfurtransferase. Other names in common use include LS, LipA, lipoate synthase, and protein 6-N-(octanoyl)lysine:sulfur sulfurtransferase. This enzyme participates in lipoic acid metabolism.

[edit] References

IUBMB entry for 2.8.1.8
BRENDA references for 2.8.1.8 (Recommended.)
PubMed references for 2.8.1.8
PubMed Central references for 2.8.1.8
Google Scholar references for 2.8.1.8
Cicchillo RM, Booker SJ (2005). "Mechanistic investigations of lipoic acid biosynthesis in Escherichia coli: both sulfur atoms in lipoic acid are contributed by the same lipoyl synthase polypeptide". J. Am. Chem. Soc. 127: 2860–1. PMID 15740115.
Vanden Boom TJ, Reed KE, Cronan JE Jr (1991). "Lipoic acid metabolism in Escherichia coli: isolation of null mutants defective in lipoic acid biosynthesis, molecular cloning and characterization of the E. coli lip locus, and identification of the lipoylated protein of the glycine cleavage system". J. Bacteriol. 173: 6411–20. PMID 1655709.
Zhao X, Miller JR, Jiang Y, Marletta MA, Cronan JE (2003). "Assembly of the covalent linkage between lipoic acid and its cognate enzymes". Chem. Biol. 10: 1293–302. PMID 14700636.
Souder MG, Tu L, Booker SJ (2004). "Lipoyl synthase requires two equivalents of S-adenosyl-L-methionine to synthesize one equivalent of lipoic acid". Biochemistry. 43: 6378–86. PMID 15157071.
Jordan SW, Cronan JE Jr (1997). "A new metabolic link. The acyl carrier protein of lipid synthesis donates lipoic acid to the pyruvate dehydrogenase complex in Escherichia coli and mitochondria". J. Biol. Chem. 272: 17903–6. PMID 9218413.
Broderick JB, Cronan JE Jr, Marletta MA (2000). "Escherichia coli LipA is a lipoyl synthase: in vitro biosynthesis of lipoylated pyruvate dehydrogenase complex from octanoyl-acyl carrier protein". Biochemistry. 39: 15166–78. PMID 11106496.
Perham RN (2000). "Swinging arms and swinging domains in multifunctional enzymes: catalytic machines for multistep reactions". Annu. Rev. Biochem. 69: 961–1004. PMID 10966480.