Linoleate diol synthase

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In enzymology, a linoleate diol synthase (EC 1.13.11.44) is an enzyme that catalyzes the chemical reaction

linoleate + O₂ $\rightleftharpoons$ (9Z,12Z)-(7S,8S)-dihydroxyoctadeca-9,12-dienoate

Thus, the two substrates of this enzyme are linoleate and O₂, whereas its product is (9Z,12Z)-(7S,8S)-dihydroxyoctadeca-9,12-dienoate.

This enzyme belongs to the family of oxidoreductases, specifically those acting on single donors with O₂ as oxidant and incorporation of two atoms of oxygen into the substrate (oxygenases). The oxygen incorporated need not be derived from O₂. The systematic name of this enzyme class is linoleate:oxygen 7S,8S-oxidoreductase. This enzyme is also called linoleate (8R)-dioxygenase. This enzyme participates in linoleic acid metabolism.

[edit] References

IUBMB entry for 1.13.11.44
BRENDA references for 1.13.11.44 (Recommended.)
PubMed references for 1.13.11.44
PubMed Central references for 1.13.11.44
Google Scholar references for 1.13.11.44
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Hamberg M, Zhang LY, Brodowsky ID, Oliw EH (1994). "Sequential oxygenation of linoleic acid in the fungus Gaeumannomyces graminis: stereochemistry of dioxygenase and hydroperoxide isomerase reactions". Arch. Biochem. Biophys. 309: 77–80. doi:10.1006/abbi.1994.1087. PMID 8117115.
EH (1999). "Cloning of linoleate diol synthase reveals homology with prostaglandin H synthases". J. Biol. Chem. 274: 28219–24. doi:10.1074/jbc.274.40.28219. PMID 10497176.
Oliw EH, Su C, Skogstrom T, Benthin G (1998). "Analysis of novel hydroperoxides and other metabolites of oleic, linoleic, and linolenic acids by liquid chromatography-mass spectrometry with ion trap MSn". Lipids. 33: 843–52. doi:10.1007/s11745-998-0280-0. PMID 9778131.
Su C, Oliw EH (1996). "Purification and characterization of linoleate 8-dioxygenase from the fungus Gaeumannomyces graminis as a novel hemoprotein". J. Biol. Chem. 271: 14112–8. doi:10.1074/jbc.271.24.14112. PMID 8662736.
Su C, Sahlin M, Oliw EH (1998). "A protein radical and ferryl intermediates are generated by linoleate diol synthase, a ferric hemeprotein with dioxygenase and hydroperoxide isomerase activities". J. Biol. Chem. 273: 20744–51. doi:10.1074/jbc.273.33.20744. PMID 9694817.