L-ribulose-5-phosphate 4-epimerase

From Wikipedia, the free encyclopedia

In enzymology, a L-ribulose-5-phosphate 4-epimerase (EC 5.1.3.4) is an enzyme that catalyzes the chemical reaction

L-ribulose 5-phosphate $\rightleftharpoons$ D-xylulose 5-phosphate

Hence, this enzyme has one substrate, L-ribulose 5-phosphate, and one product, D-xylulose 5-phosphate.

This enzyme belongs to the family of isomerases, specifically those racemases and epimerases acting on carbohydrates and derivatives. The systematic name of this enzyme class is L-ribulose-5-phosphate 4-epimerase. Other names in common use include phosphoribulose isomerase, ribulose phosphate 4-epimerase, L-ribulose-phosphate 4-epimerase, L-ribulose 5-phosphate 4-epimerase, AraD, and L-Ru5P. This enzyme participates in pentose and glucuronate interconversions and ascorbate and aldarate metabolism.

1 Structural studies
2 References
3 External links
- 3.1 Gene Ontology (GO) codes

[edit] Structural studies

As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes 1JDI and 1K0W.

[edit] References

IUBMB entry for 5.1.3.4
BRENDA references for 5.1.3.4 (Recommended.)
PubMed references for 5.1.3.4
PubMed Central references for 5.1.3.4
Google Scholar references for 5.1.3.4
BURMA DP, HORECKER BL (1958). "Not Available". J. Biol. Chem. 231: 1053–64. PMID 13539036.
Deupree JD (1970). "Wood WA: L-ribulose 5-phosphate 4-epimerase of Aerobacter aerogenes Evidence for nicotinamide adenine dinucleotide-independent 4-epimerization by the crystalline enzyme". J. Biol. Chem. 245: 3988–95. PMID 4395381.
Lee N, Patrick JW, Masson M (1968). "Crystalline L-ribulose 5-phosphate 4-epimerase from Escherichia coli". J. Biol. Chem. 243: 4700–5. PMID 4879898.
WOLIN MJ, SIMPSON FJ, WOOD WA (1958). "Degradation of L-arabinose by Aerobacter aerogenes. III Identification and properties of L-ribulose-5-phosphate 4-epimerase". J. Biol. Chem. 232: 559–75. PMID 13549442.
Andersson A, Schneider G, Lindqvist Y (1995). "Purification and preliminary X-ray crystallographic studies of recombinant L-ribulose-5-phosphate 4-epimerase from Escherichia coli". Protein. Sci. 4: 1648–50. PMID 8520491.
Lee LV, Poyner RR, Vu MV, Cleland WW (2000). "Role of metal ions in the reaction catalyzed by L-ribulose-5-phosphate 4-epimerase". Biochemistry. 39: 4821–30. doi:10.1021/bi9928952. PMID 10769139.
Samuel J, Luo Y, Morgan PM, Strynadka NC, Tanner ME (2001). "Catalysis and binding in L-ribulose-5-phosphate 4-epimerase: a comparison with L-fuculose-1-phosphate aldolase". Biochemistry. 40: 14772–80. doi:10.1021/bi011252v. PMID 11732896.