KPNB1

From Wikipedia, the free encyclopedia

Karyopherin (importin) beta 1

PDB rendering based on 1f59.

Available structures: 1f59, 1gcj, 1ibr, 1m5n, 1o6o, 1o6p, 1qgk, 1qgr, 1ukl

Identifiers

Symbol(s)

KPNB1; IMB1; IPOB; Impnb; MGC2155; MGC2156; MGC2157; NTF97

External IDs

OMIM: 602738 MGI: 107532 HomoloGene: 1707

Gene ontology
Molecular Function:	• protein binding • nuclear localization sequence binding • zinc ion binding • protein transporter activity
Cellular Component:	• nucleus • nuclear pore • cytoplasm
Biological Process:	• protein import into nucleus, docking • protein import into nucleus, translocation • NLS-bearing substrate import into nucleus • ribosomal protein import into nucleus • intracellular protein transport

RNA expression pattern

More reference expression data

Orthologs

Human

Mouse

Refseq

NM_002265 (mRNA)
NP_002256 (protein)

NM_008379 (mRNA)
NP_032405 (protein)

Location

Chr 17: 43.08 - 43.12 Mb

Chr 11: 96.98 - 97 Mb

Pubmed search

[1]

[2]

Karyopherin (importin) beta 1, also known as KPNB1, is a human gene.

Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family.^[1]

[edit] References

^ Entrez Gene: KPNB1 karyopherin (importin) beta 1.

[edit] Further reading

Bukrinsky MI, Haffar OK (2004). "HIV-1 nuclear import: in search of a leader.". Front. Biosci. 2: d578–87. PMID 9366553.
Bukrinsky MI, Haffar OK (1998). "HIV-1 nuclear import: matrix protein is back on center stage, this time together with Vpr.". Mol. Med. 4 (3): 138–43. PMID 9562972.
Christophe D, Christophe-Hobertus C, Pichon B (2000). "Nuclear targeting of proteins: how many different signals?". Cell. Signal. 12 (5): 337–41. PMID 10822175.
Chook YM, Blobel G (2002). "Karyopherins and nuclear import.". Curr. Opin. Struct. Biol. 11 (6): 703–15. PMID 11751052.
Li L, Li HS, Pauza CD, et al. (2006). "Roles of HIV-1 auxiliary proteins in viral pathogenesis and host-pathogen interactions.". Cell Res. 15 (11-12): 923–34. doi:10.1038/sj.cr.7290370. PMID 16354571.
Bukrinsky MI, Sharova N, Dempsey MP, et al. (1992). "Active nuclear import of human immunodeficiency virus type 1 preintegration complexes.". Proc. Natl. Acad. Sci. U.S.A. 89 (14): 6580–4. PMID 1631159.
Chi NC, Adam EJ, Adam SA (1995). "Sequence and characterization of cytoplasmic nuclear protein import factor p97.". J. Cell Biol. 130 (2): 265–74. PMID 7615630.
Görlich D, Kostka S, Kraft R, et al. (1995). "Two different subunits of importin cooperate to recognize nuclear localization signals and bind them to the nuclear envelope.". Curr. Biol. 5 (4): 383–92. PMID 7627554.
Bukrinsky MI, Haggerty S, Dempsey MP, et al. (1993). "A nuclear localization signal within HIV-1 matrix protein that governs infection of non-dividing cells.". Nature 365 (6447): 666–9. doi:10.1038/365666a0. PMID 8105392.
Lounsbury KM, Richards SA, Perlungher RR, Macara IG (1996). "Ran binding domains promote the interaction of Ran with p97/beta-karyopherin, linking the docking and translocation steps of nuclear import.". J. Biol. Chem. 271 (5): 2357–60. PMID 8576188.
Görlich D, Henklein P, Laskey RA, Hartmann E (1996). "A 41 amino acid motif in importin-alpha confers binding to importin-beta and hence transit into the nucleus.". EMBO J. 15 (8): 1810–7. PMID 8617226.
Weis K, Ryder U, Lamond AI (1996). "The conserved amino-terminal domain of hSRP1 alpha is essential for nuclear protein import.". EMBO J. 15 (8): 1818–25. PMID 8617227.
Moroianu J, Blobel G, Radu A (1996). "Nuclear protein import: Ran-GTP dissociates the karyopherin alphabeta heterodimer by displacing alpha from an overlapping binding site on beta.". Proc. Natl. Acad. Sci. U.S.A. 93 (14): 7059–62. PMID 8692944.
Görlich D, Panté N, Kutay U, et al. (1996). "Identification of different roles for RanGDP and RanGTP in nuclear protein import.". EMBO J. 15 (20): 5584–94. PMID 8896452.
Chi NC, Adam EJ, Adam SA (1997). "Different binding domains for Ran-GTP and Ran-GDP/RanBP1 on nuclear import factor p97.". J. Biol. Chem. 272 (10): 6818–22. PMID 9045717.
Percipalle P, Clarkson WD, Kent HM, et al. (1997). "Molecular interactions between the importin alpha/beta heterodimer and proteins involved in vertebrate nuclear protein import.". J. Mol. Biol. 266 (4): 722–32. doi:10.1006/jmbi.1996.0801. PMID 9102465.
Kutay U, Izaurralde E, Bischoff FR, et al. (1997). "Dominant-negative mutants of importin-beta block multiple pathways of import and export through the nuclear pore complex.". EMBO J. 16 (6): 1153–63. doi:10.1093/emboj/16.6.1153. PMID 9135132.
Bonifaci N, Moroianu J, Radu A, Blobel G (1997). "Karyopherin beta2 mediates nuclear import of a mRNA binding protein.". Proc. Natl. Acad. Sci. U.S.A. 94 (10): 5055–60. PMID 9144189.
Görlich D, Dabrowski M, Bischoff FR, et al. (1997). "A novel class of RanGTP binding proteins.". J. Cell Biol. 138 (1): 65–80. PMID 9214382.
Tiganis T, Flint AJ, Adam SA, Tonks NK (1997). "Association of the T-cell protein tyrosine phosphatase with nuclear import factor p97.". J. Biol. Chem. 272 (34): 21548–57. PMID 9261175.