Talk:Knockout mouse
From Wikipedia, the free encyclopedia
 |
This article is part of WikiProject Genetics, an attempt to build a comprehensive and detailed guide to genetics on Wikipedia. If you would like to participate, you can edit this page, or visit the project page to join the project and/or contribute to the discussion. |
| ??? | This article has not yet received a rating on the quality scale. |
| ??? | This article has not yet received an importance rating. |
 |
This article was previously a Medicine Collaboration of the Week. |
 |
This article is within the scope of the Molecular and Cellular Biology WikiProject. To participate, visit the WikiProject for more information. The WikiProject's current monthly collaboration is focused on improving Restriction enzyme. |
||
| Start | This article has been rated as start-Class on the assessment scale. |
||
| Mid | This article is on a subject of Mid-importance within molecular and cellular biology. | ||
|
Article Grading: The following comments were left by the quality and importance raters: (edit · history · refresh · how to use this template) Topic of 2007 Nobel Prize in Medicine - tameeria (talk) 04:54, 11 December 2007 (UTC) |
|||
[edit] resistance to antiobiotic - mistake?
In step 1 of procedure section:
a gene that normal mice don't have and that transfers resistance to a certain antibiotic
Mice aren't sensitive to antibiotics - has this bit been copied in error from an article on bacterial gene knockout? RupertMillard (Talk) 15:46, 14 July 2007 (UTC)
- it's not too well-explained, but it's not a mistake. antibiotics like Neomycin and G418 are used as selectable markers because they also kill eukaryotic cells (including mouse embryonic stem cells which are used for this procedure). i've also always been confused by the fact that they're called antibiotics but that's what people use and i can tell you first-hand that they kill mammalian cells too. the gene usually used is neomycin phosphotransferase which allows the cells to metabolize the antibiotic and thereby confers resistance. Roadnottaken 16:18, 14 July 2007 (UTC)
- That was quick! Thank you for answering my question. RupertMillard (Talk) 16:31, 14 July 2007 (UTC)
It needs to be clarified as it seems to contradict the article on 'antibiotics' which states: An antibiotic is a chemical compound that inhibits or abolishes the growth of microorganisms, such as bacteria, fungi, or protozoans. The term originally referred to any agent with biological activity against living organisms; however, "antibiotic" now is used to refer to substances with anti-bacterial, anti-fungal, or anti-parasitical activity. —Preceding unsigned comment added by 219.103.79.135 (talk) 21:12, 8 October 2007 (UTC)
[edit] MCOTW
Just a few quick notes. The article lacking full explanations, and no proper references. Subject is highly commercial so NPOV is going to be hard to get right... Feel we are missing some sections ... history? Leevanjackson (talk) 23:45, 19 February 2008 (UTC)
[edit] Fancy names
KO mouse lines do NOT get fancy names. The NHGRI link provides the information that I just deleted (on Methusalah -the name of a price, not a strain- and Frantic), but is just plain wrong. Correct naming information can be found on the site of The Jackson Laboratory (http://jaxmice.jax.org/findmice/index.html). Some older spontaneous mutants got some fancy names, especially those with cerebellar mutations (staggerer, stargazer, hotfoot, etc), but these nowadays also go by more mundane names. --Crusio (talk) 08:41, 12 March 2008 (UTC)
