Ki-67 (Biology)

From Wikipedia, the free encyclopedia

Antigen identified by monoclonal antibody Ki-67

PDB rendering based on 1r21.

Available structures: 1r21, 2aff

Identifiers

Symbol(s)

MKI67; KIA; Ki-67

External IDs

OMIM: 176741 MGI: 106035 HomoloGene: 1814

Gene ontology
Molecular Function:	• nucleotide binding • protein binding • ATP binding
Cellular Component:	• nucleus • nucleolus
Biological Process:	• regulation of progression through cell cycle • cell cycle • cell proliferation

Orthologs

Human

Mouse

n/a

Refseq

NM_002417 (mRNA)
NP_002408 (protein)

XM_001000692 (mRNA)
XP_001000692 (protein)

Location

n/a

Chr 7: 135.53 - 135.55 Mb

Pubmed search

[1]

[2]

Antibody stain against Neurofilament (green) and Ki 67 (red) in a Mouse embryo 12.5 days after fertilization. The proliferating cells are in the ventricular zone in the neural tube and therefore colored red.

The Ki-67 protein (also known as antigen identified by monoclonal antibody Ki-67or MKI67) is a cellular marker for proliferation. It is strictly associated with cell proliferation. During the interphase, the Ki-67 antigen can be exclusively detected within the cell nucleus, whereas in mitosis most of the protein is relocated to the surface of the chromosomes. Ki-67 protein is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent from resting cells (G0). Ki-67 is an excellent marker to determine the growth fraction of a given cell population. The fraction of Ki-67-positive tumor cells (the Ki-67 labelling index) is often correlated with the clinical course of cancer. The best-studied examples in this context are carcinomas of the prostate and the breast. For these types of tumors, the prognostic value for survival and tumor recurrence have repeatedly been proven in uni- and multivariate analysis.

The Ki-67 protein was originally defined by the prototype monoclonal antibody Ki-67 ^[1], which was generated by immunizing mice with nuclei of the Hodgkin lymphoma cell line L428. The name is derived from the city of origin (Kiel) and the number of the original clone in the 96-well plate.

MIB-1 is a commonly used monoclonal antibody that detects the Ki-67 antigen. It is used in clinical applications to determine the Ki-67 labelling index. One of its primary advantages over the original Ki-67 antibody (and the reason why it has essentially supplanted the original antibody for clinical use) is that it can be used on formalin-fixed paraffin-embedded sections, after heat-mediated antigen retrieval.