Isoprene synthase

From Wikipedia, the free encyclopedia

In enzymology, an isoprene synthase (EC 4.2.3.27) is an enzyme that catalyzes the chemical reaction

dimethylallyl diphosphate isoprene + diphosphate

Hence, this enzyme has one substrate, dimethylallyl diphosphate, and two products, isoprene and diphosphate.

This enzyme belongs to the family of lyases, specifically those carbon-oxygen lyases acting on phosphates. The systematic name of this enzyme class is dimethylallyl-diphosphate diphosphate-lyase (isoprene-forming). Other names in common use include ISPC, and ISPS.

[edit] References

• IUBMB entry for 4.2.3.27
• BRENDA references for 4.2.3.27 (Recommended.)
• PubMed references for 4.2.3.27
• PubMed Central references for 4.2.3.27
• Google Scholar references for 4.2.3.27
• Silver GM, Fall R (1991). "Enzymatic Synthesis of Isoprene from Dimethylallyl Diphosphate in Aspen Leaf Extracts". Plant. Physiol. 97: 1588–1591. PMID 16668590. 
• Silver GM, Fall R (1995). "Characterization of aspen isoprene synthase, an enzyme responsible for leaf isoprene emission to the atmosphere". J. Biol. Chem. 270: 13010–6. doi:10.1074/jbc.270.22.13010. PMID 7768893. 
• Wildermuth MC, Fall R (1996). "Light-Dependent Isoprene Emission (Characterization of a Thylakoid-Bound Isoprene Synthase in Salix discolor Chloroplasts)". Plant. Physiol. 112: 171–182. PMID 12226383. 
• Schnitzler JP, Arenz R, Steinbrecher R and Lehming A (1996). "Characterization of an isoprene synthase from leaves of Quercus petraea". Bot. Acta 109: 216–221. 
• Miller B, Oschinski C, Zimmer W (2001). "First isolation of an isoprene synthase gene from poplar and successful expression of the gene in Escherichia coli". Planta. 213: 483–7. doi:10.1007/s004250100557. PMID 11506373. 
• Sivy TL, Shirk MC, Fall R (2002). "Isoprene synthase activity parallels fluctuations of isoprene release during growth of Bacillus subtilis". Biochem. Biophys. Res. Commun. 294: 71–5. doi:10.1016/S0006-291X(02)00435-7. PMID 12054742. 
• Sasaki K, Ohara K, Yazaki K (2005). "Gene expression and characterization of isoprene synthase from Populus alba". FEBS. Lett. 579: 2514–8. doi:10.1016/j.febslet.2005.03.066. PMID 15848197. 
• Schnitzler JP, Zimmer I, Bachl A, Arend M, Fromm J, Fischbach RJ (2005). "Biochemical properties of isoprene synthase in poplar (Populus x canescens)". Planta. 222: 777–86. doi:10.1007/s00425-005-0022-1. PMID 16052321. 

[edit] External links

• IUBMB entry for 4.2.3.27

• KEGG entry for 4.2.3.27

• BRENDA entry for 4.2.3.27

• NiceZyme view of 4.2.3.27

• EC2PDB: PDB structures for 4.2.3.27

• PRIAM entry for 4.2.3.27

• PUMA2 entry for 4.2.3.27

• IntEnz: Integrated Enzyme entry for 4.2.3.27

• MetaCyc entry for 4.2.3.27

• Atomic-resolution structures of enzymes belonging to this class