Isoflavone 3'-hydroxylase

From Wikipedia, the free encyclopedia

In enzymology, an isoflavone 3'-hydroxylase (EC 1.14.13.52) is an enzyme that catalyzes the chemical reaction

formononetin + NADPH + H⁺ + O₂ calycosin + NADP⁺ + H₂O

The 4 substrates of this enzyme are formononetin, NADPH, H⁺, and O₂, whereas its 3 products are calycosin, NADP⁺, and H₂O.

This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with NADH or NADPH as one donor, and incorporation of one atom o oxygen into the other donor. The systematic name of this enzyme class is formononetin,NADPH:oxygen oxidoreductase (3'-hydroxylating). This enzyme is also called isoflavone 3'-monooxygenase. This enzyme participates in isoflavonoid biosynthesis. It employs one cofactor, heme.

[edit] References

• IUBMB entry for 1.14.13.52
• BRENDA references for 1.14.13.52 (Recommended.)
• PubMed references for 1.14.13.52
• PubMed Central references for 1.14.13.52
• Google Scholar references for 1.14.13.52
• Hinderer W, Flentje U and Barz W (1987). "Microsomal isoflavone 2'-hydroxylases and 3'-hydroxylases from chickpea (Cicer arietinum L) cell-suspensions induced for pterocarpan phytoalexin formation". FEBS Lett. 214: 101–106. doi:10.1016/0014-5793(87)80021-2. 

[edit] External links

The CAS registry number for this enzyme class is 110183-50-1.

• IUBMB entry for 1.14.13.52

• KEGG entry for 1.14.13.52

• BRENDA entry for 1.14.13.52

• NiceZyme view of 1.14.13.52

• EC2PDB: PDB structures for 1.14.13.52

• PRIAM entry for 1.14.13.52

• PUMA2 entry for 1.14.13.52

• IntEnz: Integrated Enzyme entry for 1.14.13.52

• MetaCyc entry for 1.14.13.52

• Atomic-resolution structures of enzymes belonging to this class

[edit] Gene Ontology (GO) codes

• EGO entry for GO code 0048000: isoflavone 3'-hydroxylase

• AMIGO entry for GO code 0048000: isoflavone 3'-hydroxylase