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hi All i am in peak to do an Inverse PCR to know the gene of my interest next the P-Gal4 insertion. I am unable to amplify the ligated fragment using T3 and T7 primers. and I have observed that most of the workers with this iPCr for the said P element they use different available Primers. So My quest is to know is there any problem to use T3 and T7 primers in iPCR after digesting with Pst-I and ligating. please share your knowledge on venkateshdevbio@gmail.com.