Infrared multiphoton dissociation
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| Infrared multiphoton dissociation | |
| Acronym | IRMPD |
|---|---|
| Other Techniques | |
| Related | Blackbody infrared radiative dissociation Electron capture dissociation Collision-induced dissociation |
Infrared multiphoton dissociation (IRMPD) is a technique used in mass spectrometry to fragment molecules in the gas phase usually for structural analysis of the original (parent) molecule. [1]
[edit] How it works
An infrared laser is directed through a window into the vacuum of the mass spectrometer where the ions are. The mechanism of fragmentation involves the absorption by a given ion of multiple infrared photons. The parent ion becomes excited into more energetic vibrational states until a bond(s) is broken resulting in gas phase fragments of the parent ion.
IRMPD is most often used in Fourier transform ion cyclotron resonance mass spectrometry.[2]
[edit] See also
- Infrared spectroscopy
- Blackbody infrared radiative dissociation
- Electron capture dissociation
- Collision-induced dissociation is another, more common, method to fragment gas phase ions.
[edit] References
- ^ Little DP, Speir JP, Senko MW, O'Connor PB, McLafferty FW (1994). "Infrared multiphoton dissociation of large multiply charged ions for biomolecule sequencing". Anal. Chem. 66 (18): 2809–15. PMID 7526742.
- ^ Laskin J, Futrell JH (2005). "Activation of large ions in FT-ICR mass spectrometry". Mass spectrometry reviews 24 (2): 135–67. doi:. PMID 15389858.
