Indo-1
From Wikipedia, the free encyclopedia
| Indo-1 | |
|---|---|
 |
|
| IUPAC name | 2-[4-(bis(carboxymethyl)amino)-3-[2-[2-(bis(carboxymethyl)amino)-
5-methylphenoxy]ethoxy]phenyl]-1H-indole-6-carboxylic acid |
| Other names | Indo-1 |
| Identifiers | |
| CAS number | [96314-96-4] |
| PubChem | |
| SMILES | CC1=CC(=C(C=C1)N(CC(=O)O)CC(=O)O)OCCOC2=C(C=CC(=C2)C3=CC4=C(N3)C=C(C=C4)
C(=O)O)N(CC(=O)O)CC(=O)O |
| Properties | |
| Molecular formula | C32H31N3O12 |
| Molar mass | 649.60 g/mol |
| Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa) Infobox disclaimer and references |
|
Indo-1 is a popular calcium indicator similar to Fura-2. In contrast to Fura-2, Indo-1 has a dual emissions peak. The main emission peak in calcium free solution is 475 nm while in the presence of calcium the the emission is shifted to 400 nm. It is widely used in flow cytometry. The penta potassium salt is commercially available and preferred to the free acid because of its higher solubility in water. While Indo-1 is not cell permeable the penta acetoxymethyl ester Indo-1 AM enters the cell where it is cleaved by intracellular esterases to Indo-1. The synthesis and properties of Indo-1 were presented in 1985 by the group of Roger Y Tsien.[1]
[edit] See also
[edit] External links
[edit] References
- ^ Grynkiewicz G, Poenie M, Tsien RY (1985). "A new generation of Ca2+ indicators with greatly improved fluorescence properties". J. Biol. Chem. 260 (6): 3440–50. PMID 3838314.
 |
This biochemistry article is a stub. You can help Wikipedia by expanding it. |
