Hygromycin B
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Hygromycin B
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Systematic (IUPAC) name | |
O-6-Amino-6-deoxy-L-glycero- D-galacto- heptopyranosylidene- (1-2-3)-O-beta-D-talopyranosyl (1-5) -2-deoxy-N3-methyl-D-streptamine | |
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ATC code | ? |
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Chemical data | |
Formula | C20H37N3O13 |
Mol. mass | 527.53 g/mol (563.5 with HCl) |
Pharmacokinetic data | |
Bioavailability | ? |
Metabolism | ? |
Half life | ? |
Excretion | ? |
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Hygromycin B, is an antibiotic produced by the bacterium Streptomyces hygroscopicus. It is an aminoglycoside that kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis[1].
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[edit] History
Hygromycin B was originally developed in the 1950's for use with animals and is still added into swine and chicken feed as an anthelmintic or anti-worming agent (product name: Hygromix). Hygromycin B is produced by Streptomyces hygroscopicus, a bacterium isolated in 1953 from a soil sample. Resistance genes were discovered in the early 1980's.[2][3]
[edit] Use in research
In the laboratory it is used for the selection and maintenance of prokaryotic and eukaryotic cells that contain the hygromycin resistance gene. The resistance gene is a kinase that inactivates hygromycin B through phosphorylation.[4] Since the discovery of hygromycin-resistance genes, hygromycin B has become a standard selection antibiotic in gene transfer experiments in many prokaryotic and eukaryotic cells.
[edit] External links
Information from hygromycin.net (InvivoGen)
[edit] References
- ^ McGuire, Pettinger (1953), “Hygromycin I. Preliminary studies on the production and biological activity of a new antibiotic.”, Antibiot. Chemother. 3: 1268-1278
- ^ Davies, Gritz (1983), “Plasmid-encoded hygromycin B resistance: the sequence of hygromycin B phosphotransferase gene and its expression in Escherichia coli and Saccharomyces cerevisiae.”, Gene 25: 179-88
- ^ Burgett, Kaster (1983), “Analysis of a bacterial hygromycin B resistance gene by transcriptional and translational fusions and by DNA sequencing.”, Nucleic Acids Res. 11: 6895-911
- ^ Rao RN, Allen NE, Hobbs JN, Alborn WE, Kirst HA, Paschal JW (1983). "Genetic and enzymatic basis of hygromycin B resistance in Escherichia coli". Antimicrobial Agents and Chemotherapy 24 (5): 689–95. PMID 6318654.