Hydroxyisourate hydrolase

From Wikipedia, the free encyclopedia

In enzymology, a hydroxyisourate hydrolase (EC 3.5.2.17) is an enzyme that catalyzes the chemical reaction

5-hydroxyisourate + H₂O $\rightleftharpoons$ 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate

Thus, the two substrates of this enzyme are 5-hydroxyisourate and H₂O, whereas its product is 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate.

This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in cyclic amides. The systematic name of this enzyme class is 5-hydroxyisourate amidohydrolase. Other names in common use include HIUHase, and 5-hydroxyisourate hydrolase. This enzyme participates in purine metabolism.

[edit] Structural studies

As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes 2H1X and 2H6U.

[edit] References

IUBMB entry for 3.5.2.17
BRENDA references for 3.5.2.17 (Recommended.)
PubMed references for 3.5.2.17
PubMed Central references for 3.5.2.17
Google Scholar references for 3.5.2.17
Raychaudhuri A, Tipton PA (2003). "A familiar motif in a new context: the catalytic mechanism of hydroxyisourate hydrolase". Biochemistry. 42: 6848–52. doi:10.1021/bi034137o. PMID 12779339.
Raychaudhuri A, Tipton PA (2002). "Cloning and expression of the gene for soybean hydroxyisourate hydrolase. Localization and implications for function and mechanism". Plant. Physiol. 130: 2061–8. doi:10.1104/pp.011049. PMID 12481089.
Sarma AD, Serfozo P, Kahn K, Tipton PA (1999). "Identification and purification of hydroxyisourate hydrolase, a novel ureide-metabolizing enzyme". J. Biol. Chem. 274: 33863–5. doi:10.1074/jbc.274.48.33863. PMID 10567345.