Hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolysing)

From Wikipedia, the free encyclopedia

In enzymology, a hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolysing) (EC 6.3.5.9) is an enzyme that catalyzes the chemical reaction

2 ATP + hydrogenobyrinic acid + 2 L-glutamine + 2 H₂O $\rightleftharpoons$ 2 ADP + 2 phosphate + hydrogenobyrinic acid a,c-diamide + 2 L-glutamate

The 4 substrates of this enzyme are ATP, hydrogenobyrinic acid, L-glutamine, and H₂O, whereas its 4 products are ADP, phosphate, hydrogenobyrinic acid a,c-diamide, and L-glutamate.

This enzyme belongs to the family of ligases, specifically those forming carbon-nitrogen bonds carbon-nitrogen ligases with glutamine as amido-N-donor. The systematic name of this enzyme class is hydrogenobyrinic-acid:L-glutamine amido-ligase (AMP-forming). This enzyme is also called CobB. This enzyme participates in porphyrin and chlorophyll metabolism.

[edit] References

IUBMB entry for 6.3.5.9
BRENDA references for 6.3.5.9 (Recommended.)
PubMed references for 6.3.5.9
PubMed Central references for 6.3.5.9
Google Scholar references for 6.3.5.9
Debussche L, Thibaut D, Cameron B, Crouzet J, Blanche F (1990). "Purification and characterization of cobyrinic acid a,c-diamide synthase from Pseudomonas denitrificans". J. Bacteriol. 172: 6239–44. PMID 2172209.
Warren MJ, Raux E, Schubert HL, Escalante-Semerena JC (2002). "The biosynthesis of adenosylcobalamin (vitamin B12)". Nat. Prod. Rep. 19: 390–412. doi:10.1039/b108967f. PMID 12195810.