HUS1

From Wikipedia, the free encyclopedia


HUS1 checkpoint homolog (S. pombe)
Identifiers
Symbol(s) HUS1;
External IDs OMIM: 603760 MGI: 1277962 HomoloGene: 37932
RNA expression pattern

More reference expression data
Orthologs
Human Mouse
Entrez 3364 15574
Ensembl ENSG00000136273 ENSMUSG00000020413
Uniprot O60921 Q8BQY8
Refseq NM_004507 (mRNA)
NP_004498 (protein)		 NM_008316 (mRNA)
NP_032342 (protein)
Location Chr 7: 47.97 - 47.99 Mb Chr 11: 8.89 - 8.91 Mb
Pubmed search [1] [2]

HUS1 checkpoint homolog (S. pombe), also known as HUS1, is a human gene.

The protein encoded by this gene is a component of an evolutionarily conserved, genotoxin-activated checkpoint complex that is involved in the cell cycle arrest in response to DNA damage. This protein forms a heterotrimeric complex with checkpoint proteins RAD9 and RAD1. In response to DNA damage, the trimeric complex interacts with another protein complex consisting of checkpoint protein RAD17 and four small subunits of the replication factor C (RFC), which loads the combined complex onto the chromatin. The DNA damage induced chromatin binding has been shown to depend on the activation of the checkpoint kinase ATM, and is thought to be an early checkpoint signaling event.[1]

[edit] References

  1. ^ Entrez Gene: HUS1 HUS1 checkpoint homolog (S. pombe).

[edit] Further reading

  • Kostrub CF, Knudsen K, Subramani S, Enoch T (1998). "Hus1p, a conserved fission yeast checkpoint protein, interacts with Rad1p and is phosphorylated in response to DNA damage.". EMBO J. 17 (7): 2055–66. doi:10.1093/emboj/17.7.2055. PMID 9524127. 
  • Dominguez O, Ashhab Y, Sabater L, et al. (1999). "Cloning of ARE-containing genes by AU-motif-directed display.". Genomics 54 (2): 278–86. doi:10.1006/geno.1998.5548. PMID 9828130. 
  • Volkmer E, Karnitz LM (1999). "Human homologs of Schizosaccharomyces pombe rad1, hus1, and rad9 form a DNA damage-responsive protein complex.". J. Biol. Chem. 274 (2): 567–70. PMID 9872989. 
  • Dean FB, Lian L, O'Donnell M (1999). "cDNA cloning and gene mapping of human homologs for Schizosaccharomyces pombe rad17, rad1, and hus1 and cloning of homologs from mouse, Caenorhabditis elegans, and Drosophila melanogaster.". Genomics 54 (3): 424–36. doi:10.1006/geno.1998.5587. PMID 9878245. 
  • St Onge RP, Udell CM, Casselman R, Davey S (1999). "The human G2 checkpoint control protein hRAD9 is a nuclear phosphoprotein that forms complexes with hRAD1 and hHUS1.". Mol. Biol. Cell 10 (6): 1985–95. PMID 10359610. 
  • Hang H, Lieberman HB (2000). "Physical interactions among human checkpoint control proteins HUS1p, RAD1p, and RAD9p, and implications for the regulation of cell cycle progression.". Genomics 65 (1): 24–33. doi:10.1006/geno.2000.6142. PMID 10777662. 
  • Cai RL, Yan-Neale Y, Cueto MA, et al. (2000). "HDAC1, a histone deacetylase, forms a complex with Hus1 and Rad9, two G2/M checkpoint Rad proteins.". J. Biol. Chem. 275 (36): 27909–16. doi:10.1074/jbc.M000168200. PMID 10846170. 
  • Burtelow MA, Kaufmann SH, Karnitz LM (2000). "Retention of the human Rad9 checkpoint complex in extraction-resistant nuclear complexes after DNA damage.". J. Biol. Chem. 275 (34): 26343–8. doi:10.1074/jbc.M001244200. PMID 10852904. 
  • Rauen M, Burtelow MA, Dufault VM, Karnitz LM (2000). "The human checkpoint protein hRad17 interacts with the PCNA-like proteins hRad1, hHus1, and hRad9.". J. Biol. Chem. 275 (38): 29767–71. doi:10.1074/jbc.M005782200. PMID 10884395. 
  • Komatsu K, Wharton W, Hang H, et al. (2000). "PCNA interacts with hHus1/hRad9 in response to DNA damage and replication inhibition.". Oncogene 19 (46): 5291–7. doi:10.1038/sj.onc.1203901. PMID 11077446. 
  • Xiang SL, Kumano T, Iwasaki SI, et al. (2001). "The J domain of Tpr2 regulates its interaction with the proapoptotic and cell-cycle checkpoint protein, Rad9.". Biochem. Biophys. Res. Commun. 287 (4): 932–40. doi:10.1006/bbrc.2001.5685. PMID 11573955. 
  • Griffith JD, Lindsey-Boltz LA, Sancar A (2002). "Structures of the human Rad17-replication factor C and checkpoint Rad 9-1-1 complexes visualized by glycerol spray/low voltage microscopy.". J. Biol. Chem. 277 (18): 15233–6. doi:10.1074/jbc.C200129200. PMID 11907025. 
  • Kinzel B, Hall J, Natt F, et al. (2002). "Downregulation of Hus1 by antisense oligonucleotides enhances the sensitivity of human lung carcinoma cells to cisplatin.". Cancer 94 (6): 1808–14. PMID 11920544. 
  • Hang H, Zhang Y, Dunbrack RL, et al. (2002). "Identification and characterization of a paralog of human cell cycle checkpoint gene HUS1.". Genomics 79 (4): 487–92. doi:10.1006/geno.2002.6737. PMID 11944979. 
  • Roos-Mattjus P, Vroman BT, Burtelow MA, et al. (2003). "Genotoxin-induced Rad9-Hus1-Rad1 (9-1-1) chromatin association is an early checkpoint signaling event.". J. Biol. Chem. 277 (46): 43809–12. doi:10.1074/jbc.M207272200. PMID 12228248. 
  • Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMID 12477932. 
  • Bermudez VP, Lindsey-Boltz LA, Cesare AJ, et al. (2003). "Loading of the human 9-1-1 checkpoint complex onto DNA by the checkpoint clamp loader hRad17-replication factor C complex in vitro.". Proc. Natl. Acad. Sci. U.S.A. 100 (4): 1633–8. doi:10.1073/pnas.0437927100. PMID 12578958. 
  • Scherer SW, Cheung J, MacDonald JR, et al. (2003). "Human chromosome 7: DNA sequence and biology.". Science 300 (5620): 767–72. doi:10.1126/science.1083423. PMID 12690205. 
  • Hopkins KM, Wang X, Berlin A, et al. (2003). "Expression of mammalian paralogues of HRAD9 and Mrad9 checkpoint control genes in normal and cancerous testicular tissue.". Cancer Res. 63 (17): 5291–8. PMID 14500360. 
  • Dufault VM, Oestreich AJ, Vroman BT, Karnitz LM (2004). "Identification and characterization of RAD9B, a paralog of the RAD9 checkpoint gene.". Genomics 82 (6): 644–51. PMID 14611806. 
 This article on a gene on chromosome 7 is a stub. You can help Wikipedia by expanding it.