Hot 100 number-one hits of 1965 (United States)
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These are the Billboard magazine Hot 100 number one hits of 1965.
| Issue Date | Song | Artist |
| January 2 | "I Feel Fine" | The Beatles |
| January 9 | "I Feel Fine" | The Beatles |
| January 16 | "Come See About Me" | The Supremes |
| January 23 | "Downtown" | Petula Clark |
| January 30 | "Downtown" | Petula Clark |
| February 6 | "You've Lost That Lovin' Feelin'" | The Righteous Brothers |
| February 13 | "You've Lost That Lovin' Feelin'" | The Righteous Brothers |
| February 20 | "This Diamond Ring" | Gary Lewis & the Playboys |
| February 27 | "This Diamond Ring" | Gary Lewis & The Playboys |
| March 6 | "My Girl" | The Temptations |
| March 13 | "Eight Days a Week" | The Beatles |
| March 20 | "Eight Days a Week" | The Beatles |
| March 27 | "Stop! In the Name of Love" | The Supremes |
| April 3 | "Stop! In the Name of Love" | The Supremes |
| April 10 | "I'm Telling You Now" | Freddie and the Dreamers |
| April 17 | "I'm Telling You Now" | Freddie and the Dreamers |
| April 24 | "Game of Love" | Wayne Fontana and the Mindbenders |
| May 1 | "Mrs. Brown, You've Got a Lovely Daughter" | Herman's Hermits |
| May 8 | "Mrs. Brown, You've Got a Lovely Daughter" | Herman's Hermits |
| May 15 | "Mrs. Brown, You've Got a Lovely Daughter" | Herman's Hermits |
| May 22 | "Ticket to Ride" | The Beatles |
| May 29 | "Help Me Rhonda" | The Beach Boys |
| June 5 | "Help Me Rhonda" | The Beach Boys |
| June 12 | "Back in My Arms Again" | The Supremes |
| June 19 | "I Can't Help Myself (Sugar Pie Honey Bunch)" | The Four Tops |
| June 26 | "Mr. Tambourine Man" | The Byrds |
| July 3 | "I Can't Help Myself (Sugar Pie Honey Bunch)" | The Four Tops |
| July 10 | "(I Can't Get No) Satisfaction" | The Rolling Stones |
| July 17 | "(I Can't Get No) Satisfaction" | The Rolling Stones |
| July 24 | "(I Can't Get No) Satisfaction" | The Rolling Stones |
| July 31 | "(I Can't Get No) Satisfaction" | The Rolling Stones |
| August 7 | "I'm Henry VIII, I Am" | Herman's Hermits |
| August 14 | "I Got You Babe" | Sonny and Cher |
| August 21 | "I Got You Babe" | Sonny and Cher |
| August 28 | "I Got You Babe" | Sonny and Cher |
| September 4 | "Help!" | The Beatles |
| September 11 | "Help!" | The Beatles |
| September 18 | "Help!" | The Beatles |
| September 25 | "Eve of Destruction" | Barry McGuire |
| October 2 | "Hang on Sloopy" | The McCoys |
| October 9 | "Yesterday" | The Beatles |
| October 16 | "Yesterday" | The Beatles |
| October 23 | "Yesterday" | The Beatles |
| October 30 | "Yesterday" | The Beatles |
| November 6 | "Get Off of My Cloud" | The Rolling Stones |
| November 13 | "Get Off of My Cloud" | The Rolling Stones |
| November 20 | "I Hear a Symphony" | The Supremes |
| November 27 | "I Hear a Symphony" | The Supremes |
| December 4 | "Turn! Turn! Turn! (To Everything There Is a Season)" | The Byrds |
| December 11 | "Turn! Turn! Turn! (To Everything There Is a Season)" | The Byrds |
| December 18 | "Turn! Turn! Turn! (To Everything There Is a Season)" | The Byrds |
| December 25 | "Over and Over" | The Dave Clark Five |
