Glycoprotein 2-beta-D-xylosyltransferase

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In enzymology, a glycoprotein 2-beta-D-xylosyltransferase (EC 2.4.2.38) is an enzyme that catalyzes the chemical reaction

UDP-D-xylose + N₄-{N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N- acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-beta-D- mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D- glucosaminyl}asparagine $\rightleftharpoons$ UDP + N₄-{N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N- acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-[beta-D- xylosyl-(1->2)]-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl- (1->4)-N-acetyl-beta-D-glucosaminyl}asparagine

The 5 substrates of this enzyme are UDP-D-xylose, [[N4-{N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N-]], [[acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-beta-D-]], [[mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-]], and [[glucosaminyl}asparagine]], whereas its 5 products are UDP, [[N4-{N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N-]], [[acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-[beta-D-]], [[xylosyl-(1->2)]-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-]], and [[(1->4)-N-acetyl-beta-D-glucosaminyl}asparagine]].

This enzyme belongs to the family of glycosyltransferases, specifically the pentosyltransferases. The systematic name of this enzyme class is UDP-D-xylose:glycoprotein (D-xylose to the 3,6-disubstituted mannose of N4-{N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N-a cetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-beta-D-man nosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glu cosaminyl}asparagine) 2-beta-D-xylosyltransferase. Other names in common use include beta1,2-xylosyltransferase, UDP-D-xylose:glycoprotein (D-xylose to the 3,6-disubstituted mannose, of, 4-N-{N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N-, acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-beta-D-, mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-, and glucosaminyl}asparagine) 2-beta-D-xylosyltransferase. This enzyme participates in glycan structures - biosynthesis 1.

[edit] References

IUBMB entry for 2.4.2.38
BRENDA references for 2.4.2.38 (Recommended.)
PubMed references for 2.4.2.38
PubMed Central references for 2.4.2.38
Google Scholar references for 2.4.2.38
Zeng Y, Bannon G, Thomas VH, Rice K, Drake R, Elbein A (1997). "Purification and specificity of beta1,2-xylosyltransferase, an enzyme that contributes to the allergenicity of some plant proteins". J. Biol. Chem. 272: 31340–7. doi:10.1074/jbc.272.50.31340. PMID 9395463.
Steinkellner H (2000). "Molecular cloning and functional expression of beta1, 2-xylosyltransferase cDNA from Arabidopsis thaliana". FEBS. Lett. 472: 105–8. doi:10.1016/S0014-5793(00)01443-5. PMID 10781814.