Germacrene-A synthase

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In enzymology, a germacrene-A synthase (EC 4.2.3.23) is an enzyme that catalyzes the chemical reaction

2-trans,6-trans-farnesyl diphosphate $\rightleftharpoons$ (+)-(R)-gemacrene A + diphosphate

Hence, this enzyme has one substrate, 2-trans,6-trans-farnesyl diphosphate, and two products, (+)-(R)-gemacrene A and diphosphate.

This enzyme belongs to the family of lyases, specifically those carbon-oxygen lyases acting on phosphates. The systematic name of this enzyme class is 2-trans,6-trans-farnesyl-diphosphate diphosphate-lyase [(+)-(R)-germacrene-A-forming]. Other names in common use include germacrene A synthase, (+)-germacrene A synthase, (+)-(10R)-germacrene A synthase, GAS, 2-trans,6-trans-farnesyl-diphosphate diphosphate-lyase, and (germacrene-A-forming).

[edit] References

IUBMB entry for 4.2.3.23
BRENDA references for 4.2.3.23 (Recommended.)
PubMed references for 4.2.3.23
PubMed Central references for 4.2.3.23
Google Scholar references for 4.2.3.23
Wallaart TE (2002). "Isolation and characterization of two germacrene A synthase cDNA clones from chicory". Plant. Physiol. 129: 134–44. doi:10.1104/pp.001024. PMID 12011345.
Beale MH (2002). "(+)-(10R)-Germacrene A synthase from goldenrod, Solidago canadensis cDNA isolation, bacterial expression and functional analysis". Phytochemistry. 60: 691–702. doi:10.1016/S0031-9422(02)00165-6. PMID 12127586.
de Kraker JW, Franssen MC, de Groot A, Konig WA, Bouwmeester HJ (1998). "(+)-Germacrene A biosynthesis . The committed step in the biosynthesis of bitter sesquiterpene lactones in chicory". Plant. Physiol. 117: 1381–92. doi:10.1104/pp.117.4.1381. PMID 9701594.
Calvert MJ, Ashton PR, Allemann RK (2002). "Germacrene A is a product of the aristolochene synthase-mediated conversion of farnesylpyrophosphate to aristolochene". J. Am. Chem. Soc. 124: 11636–41. doi:10.1021/ja020762p. PMID 12296728.
Chang YJ, Jin J, Nam HY, Kim SU (2005). "Point mutation of (+)-germacrene A synthase from Ixeris dentata". Biotechnol. Lett. 27: 285–8. doi:10.1007/s10529-005-0681-9. PMID 15834787.