Gene Knock-in

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In molecular cloning and biology, a Knock-in (or Gene knock-in) refers to a genetic engineering method that involves the insertion of a protein coding cDNA sequence at a particular locus in an organism's chromosome. Typically, this is done using a murine (mouse) model as they are easy to breed and exhibit relatively short development. It is a technique by which scientific investigators may study the function of the regulatory machinery (e.g promotors) that governs the expression of the natural gene being replaced. This is accomplished by observing the new phenotype of the organism in question. This technique is essentially the opposite of a gene knockout.