GDP-mannose 4,6-dehydratase

From Wikipedia, the free encyclopedia

In enzymology, a GDP-mannose 4,6-dehydratase (EC 4.2.1.47) is an mannosyltransferase enzyme that catalyzes the chemical reaction

GDP-mannose $\rightleftharpoons$ GDP-4-dehydro-6-deoxy-D-mannose + H₂O

Hence, this enzyme has one substrate, GDP-mannose, and two products, GDP-4-dehydro-6-deoxy-D-mannose and H₂O.

This enzyme belongs to the family of lyases, specifically the hydro-lyases, which cleave carbon-oxygen bonds. The systematic name of this enzyme class is GDP-mannose 4,6-hydro-lyase (GDP-4-dehydro-6-deoxy-D-mannose-forming). Other names in common use include guanosine 5'-diphosphate-D-mannose oxidoreductase, guanosine diphosphomannose oxidoreductase, guanosine diphosphomannose 4,6-dehydratase, GDP-D-mannose dehydratase, GDP-D-mannose 4,6-dehydratase, Gmd, and GDP-mannose 4,6-hydro-lyase. This enzyme participates in fructose and mannose metabolism. It employs one cofactor, NAD+.

1 Structural studies
2 References
3 External links
- 3.1 Gene Ontology (GO) codes

[edit] Structural studies

As of late 2007, 5 structures have been solved for this class of enzymes, with PDB accession codes 1DB3, 1N7G, 1N7H, 1RPN, and 1T2A.

[edit] References

IUBMB entry for 4.2.1.47
BRENDA references for 4.2.1.47 (Recommended.)
PubMed references for 4.2.1.47
PubMed Central references for 4.2.1.47
Google Scholar references for 4.2.1.47
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Liao TH, Barber GA (1972). "Purification of guanosine 5'-diphosphate D-mannose oxidoreductase from Phaseolus vulgaris". Biochim. Biophys. Acta. 276: 85–93. PMID 5047712.
Melo A, Elliott WH and Glaser L (1968). "The mechanism of 6-deoxyhexose synthesis. I. Intramolecular hydrogen transfer catalyzed by deoxythymidine diphosphate D-glucose oxidoreductase". J. Biol. Chem. 243: 1467–74. PMID 4869560.
Sullivan FX, Kumar R, Kriz R, Stahl M, Xu GY, Rouse J, Chang XJ, Boodhoo A, Potvin B and Cumming DA (1998). "Molecular cloning of human GDP-mannose 4,6-dehydratase and reconstitution of GDP-fucose biosynthesis in vitro". J. Biol. Chem. 273: 8193–202. doi:10.1074/jbc.273.14.8193. PMID 9525924.
Kneidinger B, Graninger M, Adam G, Puchberger M, Kosma P, Zayni S and Messner P (2001). "Identification of two GDP-6-deoxy-D-lyxo-4-hexulose reductases synthesizing GDP-D-rhamnose in Aneurinibacillus thermoaerophilus L420-91T". J. Biol. Chem. 276: 5577–83. doi:10.1074/jbc.M010027200. PMID 11096116.
Mulichak AM, Bonin CP, Reiter WD and Garavito RM (2002). "Structure of the MUR1 GDP-mannose 4,6-dehydratase from Arabidopsis thaliana: implications for ligand binding and specificity". Biochemistry. 41: 15578–89. doi:10.1021/bi0266683. PMID 12501186.