Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase

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In enzymology, a galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase (EC 2.4.1.135) is an enzyme that catalyzes the chemical reaction

UDP-glucuronate + 3-beta-D-galactosyl-4-beta-D-galactosyl-O-beta-D-xylosylprotein $\rightleftharpoons$ UDP + 3-beta-D-glucuronosyl-3-beta-D-galactosyl-4-beta-D-galactosyl-O- beta-D-xylosylprotein

Thus, the two substrates of this enzyme are UDP-glucuronate and 3-beta-D-galactosyl-4-beta-D-galactosyl-O-beta-D-xylosylprotein, whereas its 3 products are UDP, 3-beta-D-glucuronosyl-3-beta-D-galactosyl-4-beta-D-galactosyl-O-, and beta-D-xylosylprotein.

This enzyme belongs to the family of glycosyltransferases, specifically the hexosyltransferases. The systematic name of this enzyme class is UDP-glucuronate:3-beta-D-galactosyl-4-beta-D-galactosyl-O-beta-D-xyl osyl-protein D-glucuronosyltransferase. Other names in common use include glucuronosyltransferase I, and uridine diphosphate glucuronic acid:acceptor glucuronosyltransferase. This enzyme participates in chondroitin sulfate biosynthesis and glycan structures - biosynthesis 1. It employs one cofactor, manganese.

1 Structural studies
2 References
3 External links
- 3.1 Gene Ontology (GO) codes

[edit] Structural studies

As of late 2007, 4 structures have been solved for this class of enzymes, with PDB accession codes 1V82, 1V83, 1V84, and 2D0J.

[edit] References

IUBMB entry for 2.4.1.135
BRENDA references for 2.4.1.135 (Recommended.)
PubMed references for 2.4.1.135
PubMed Central references for 2.4.1.135
Google Scholar references for 2.4.1.135
Helting T, Roden L (1969). "Biosynthesis of chondroitin sulfate. II. Glucuronosyl transfer in the formation of the carbohydrate-protein linkage region". J. Biol. Chem. 244: 2799–805. PMID 5770003.
Helting T (1972). "Biosynthesis of heparin. Solubilization and partial purification of uridine diphosphate glucuronic acid: acceptor glucuronosyltransferase from mouse mastocytoma". J. Biol. Chem. 247: 4327–32. PMID 4260846.
T, Sugahara K (1998). "Molecular cloning and expression of glucuronyltransferase I involved in the biosynthesis of the glycosaminoglycan-protein linkage region of proteoglycans". J. Biol. Chem. 273: 6615–8. doi:10.1074/jbc.273.12.6615. PMID 9506957.