G banding

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G-banding is technique used in cytogenetics to produce differently stained regions on condensed chromosomes.^[1] The metaphase chromosomes are treated with trypsin (to partially digest the protein) and stained with Giemsa. Dark bands that take up the stain are strongly A,T rich (gene poor). The reverse of G-bands is obtained in R-banding. Banding can be used to identity chromosomal abnormalies, such as translocations, because there is a unique pattern of light and dark bands for each chromosome.^[2]

[edit] See also

• Giemsa

[edit] References

1. ^ Speicher, Michael R. and Nigel P. Carter. "The New Cytogenetics: Blurring the Boundaries with Molecular Biology." Nature Reviews Genetics, Vol 6. Oct 2005.
2. ^ ibid.