Fura-2-acetoxymethyl ester

From Wikipedia, the free encyclopedia

Fura-2-acetoxymethyl ester
IUPAC name Acetoxymethyl 2-[5-[bis[(acetoxymethoxy-oxo-

methyl)methyl]amino]-4-[2-[2-[bis[(acetoxymethoxy-oxo- methyl)methyl]amino]-5-methyl-phenoxy]ethoxy]benzofuran- 2-yl]oxazole-5-carboxylate

Other names Fura-2AM
Identifiers
CAS number [108964-32-5]
PubChem 3086531
SMILES CC1=CC(=C(C=C1)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)

OCCOC2=C(C=CC3=C2C=C(O3)C4=NC=C(O4)C(=O)OCOC(=O)C)N (CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C

Properties
Molecular formula C44H47N3O24
Molar mass 1001.85
Except where noted otherwise, data are given for
materials in their standard state
(at 25 °C, 100 kPa)

Infobox disclaimer and references

Fura-2-acetoxymethyl ester, often abbreviated Fura-2AM, is a ratiometric calcium indicator used in biochemistry, measured by changes in fluorescence. Fura-2AM is a membrane-permeable derivative of Fura-2.

Measurement of Ca+2-induced fluorescence at both 340 nm and 380 nm allows for 340/380 ratios. This helps in cancellation of artifacts produced by changes in dye concentrations.