Fosmid

From Wikipedia, the free encyclopedia

This article may require cleanup to meet Wikipedia's quality standards.
Please improve this article if you can. (October 2006)

Fosmids are similar to cosmids but are based on the bacterial F-plasmid. The cloning vector is limited, as a host (usually E. coli) can only contain one fosmid molecule. Low copy number offers higher stability than comparable high copy number cosmids. Fosmid system may be useful for constructing stable libraries from complex genomes. Fosmid clones were used to help assess the accuracy of the Public Human Genome Sequence^[1].

[edit] References

^ Ung-Jin Kim et al. 1992. Stable propagation of cosmid-sized human DNA inserts in an F-factor based vector. Nucleic Acids Res. 20:1083-1085.

v • d • e Types of nucleic acids

Ribonucleic acids	RNA \| mRNA (pre-mRNA/hnRNA) \| tRNA \| rRNA \| aRNA \| gRNA \| miRNA \| ncRNA \| piRNA \| shRNA \| siRNA \| snRNA \| snoRNA \| tmRNA

Deoxyribonucleic acids	DNA \| cDNA \| gDNA \| msDNA \| mtDNA

Nucleic acid analogues	GNA \| LNA \| PNA \| TNA \| morpholino

Cloning vectors	phagemid \| plasmid \| lambda phage \| cosmid \| P1 phage \| fosmid \| BAC \| YAC \| HAC

Major families of biochemicals Peptides \| Amino acids \| Nucleic acids \| Carbohydrates \| Nucleotide sugars \| Lipids \| Terpenes \| Carotenoids \| Tetrapyrroles \| Enzyme cofactors \| Steroids \| Flavonoids \| Alkaloids \| Polyketides \| Glycosides