Formate-tetrahydrofolate ligase

From Wikipedia, the free encyclopedia

In enzymology, a formate-tetrahydrofolate ligase (EC 6.3.4.3) is an enzyme that catalyzes the chemical reaction

ATP + formate + tetrahydrofolate $\rightleftharpoons$ ADP + phosphate + 10-formyltetrahydrofolate

The 3 substrates of this enzyme are ATP, formate, and tetrahydrofolate, whereas its 3 products are ADP, phosphate, and 10-formyltetrahydrofolate.

This enzyme belongs to the family of ligases, specifically those forming generic carbon-nitrogen bonds. The systematic name of this enzyme class is formate:tetrahydrofolate ligase (ADP-forming). Other names in common use include formyltetrahydrofolate synthetase, 10-formyltetrahydrofolate synthetase, tetrahydrofolic formylase, and tetrahydrofolate formylase. This enzyme participates in glyoxylate and dicarboxylate metabolism and one carbon pool by folate.

1 Structural studies
2 References
3 External links
- 3.1 Gene Ontology (GO) codes

[edit] Structural studies

As of late 2007, 3 structures have been solved for this class of enzymes, with PDB accession codes 1EG7, 1FP7, and 1FPM.

[edit] References

IUBMB entry for 6.3.4.3
BRENDA references for 6.3.4.3 (Recommended.)
PubMed references for 6.3.4.3
PubMed Central references for 6.3.4.3
Google Scholar references for 6.3.4.3
JAENICKE L, BRODE E (1961). "[Research on monocarbon compounds. I. The tetrahydrofolate formylase from pigeon liver. Purification and mechanism.]". Biochem. Z. 334: 108–32. PMID 13789141.
Long CW, Levitzki A, Houston LL and Koshland DE, Jr (1969). "Subunit structures and interactions of CTP synthetase". Fed. Proc. 28: 342.
RABINOWITZ JC, PRICER WE Jr (1962). "Formyltetrahydrofolate synthetase. I. Isolation and crystallization of the enzyme". J. Biol. Chem. 237: 2898–902. PMID 14489619.
Whiteley HR, Osborn MJ and Huennekens FM (1959). "Purification and properties of the formate-activating enzyme from Micrococcus aerogenes". J. Biol. Chem. 234: 1538–1543.