Folin-Ciocalteu reagent

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The Folin-Ciocalteu reagent or Folin's phenol reagent or Folin-Denis reagent is a mixture of phosphomolybdate and phosphotungstate used for the colorimetric assay of phenolic antioxidants and polyphenol antioxidants.[1] It works by measuring the amount of the substance being tested needed to inhibit the oxidation of the reagent.[2]

However, this reagent does not only measure total phenols and will react with any reducing substance. The reagent therefore measures the total reducing capacity of a sample, not just the level of phenolic compounds. This reagent forms part of the Lowry protein assay and will also react with some nitrogen-containing compounds such as hydroxylamine and guanidine.[3]

This reagent should not be confused with Folin's reagent, which is used to detect amines and sulfur-containing compounds.

[edit] References

  1. ^ Singleton VL, Orthofer R, Lamuela-Raventos RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Meth Enzymol 1999; 299: 152-178.
  2. ^ Vinson J, Zubik L, Bose P, Samman N, Proch J (2005). "Dried fruits: excellent in vitro and in vivo antioxidants". J Am Coll Nutr 24 (1): 44–50. PMID 15670984. 
  3. ^ Ikawa M, Schaper TD, Dollard CA, Sasner JJ (2003). "Utilization of Folin-Ciocalteu phenol reagent for the detection of certain nitrogen compounds". J. Agric. Food Chem. 51 (7): 1811–5. doi:10.1021/jf021099r. PMID 12643635. 

[edit] External links


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