Talk:Fetal bovine serum

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[edit] Question

I know Wikipedia is not a forum, however I have a question (as a biologist) whose answer could possibly be added to this article. Why are cells serum-starved (eg. quite often for 24h) before an experiment? What is the effect of serum that can affect the results and conclusions of an experiment? 155.207.86.45 07:30, 6 June 2007 (UTC)


[edit] Answer

Depending on your experiment, it would appropriate to serum-starve your cells for two reasons. For one, serum starvation with some cell lines induces morphological changes. CAD cells, upon serum-starvation, differentiate into neuron-like cells. Serum starvation can also induce a stronger response in some assays. For example, some cells would show a stronger response towards growth factors for cell motility assays and growth-factor response assays.

杉村弘樹でした～！ Sugimurahiroki 08:16, 3 December 2007 (UTC)

[edit] Spamming Wikipedia

Biologicalworld.com has spammed wikipedia like no tomorrow. He is a site of only a few pages and a LOT of adsense. Not much information is given except for "protocols" which are not referenced, and cannot be trusted from a site of that quality.

check: Links from Wikipedia

The following have been cleaned up:

  • en.wikipedia.org/wiki/Plasmid
  • en.wikipedia.org/wiki/Gel_electrophoresis
  • en.wikipedia.org/wiki/Green_fluorescent_protein
  • en.wikipedia.org/wiki/Homology_(biology)
  • en.wikipedia.org/wiki/HeLa
  • en.wikipedia.org/wiki/Protease
  • en.wikipedia.org/wiki/Restriction_enzyme
  • en.wikipedia.org/wiki/Petri_dish
  • en.wikipedia.org/wiki/Structural_domain
  • en.wikipedia.org/wiki/Trypsin
  • en.wikipedia.org/wiki/Oligonucleotide
  • en.wikipedia.org/wiki/Transmission_electron_microscope
  • en.wikipedia.org/wiki/Agar_plate
  • en.wikipedia.org/wiki/Calcium_phosphate
  • en.wikipedia.org/wiki/Disulfide_bond
  • en.wikipedia.org/wiki/Denaturation_(biochemistry)
  • en.wikipedia.org/wiki/DNA_ligase
  • en.wikipedia.org/wiki/Wild_type
  • en.wikipedia.org/wiki/Tissue_culture
  • en.wikipedia.org/wiki/Transmission_electron_microscopy
  • en.wikipedia.org/wiki/Reporter_gene
  • en.wikipedia.org/wiki/Northern_blot
  • en.wikipedia.org/wiki/Protein_engineering
  • en.wikipedia.org/wiki/Sticky_end/blunt_end
  • en.wikipedia.org/wiki/Taq_polymerase
  • en.wikipedia.org/wiki/Protein_domain
  • en.wikipedia.org/wiki/Coomassie
  • en.wikipedia.org/wiki/Native_state
  • en.wikipedia.org/wiki/Chinese_Hamster_Ovary_cell
  • en.wikipedia.org/wiki/Peptidase
  • en.wikipedia.org/wiki/Visking_tubing
  • en.wikipedia.org/wiki/Streptavidin
  • en.wikipedia.org/wiki/Microtiter_plate
  • en.wikipedia.org/wiki/Subcloning
  • en.wikipedia.org/wiki/Ion_exchange_chromatography
  • en.wikipedia.org/wiki/Thermal_cycler
  • en.wikipedia.org/wiki/Bovine_serum_albumin
  • en.wikipedia.org/wiki/Phosphate_buffered_saline
  • en.wikipedia.org/wiki/Glutathione_S-transferase
  • en.wikipedia.org/wiki/HEPES
  • en.wikipedia.org/wiki/Ortholog
  • en.wikipedia.org/wiki/Proteases
  • en.wikipedia.org/wiki/Salting_out
  • en.wikipedia.org/wiki/Fetal_bovine_serum
  • en.wikipedia.org/wiki/Proteolytic_enzyme
  • en.wikipedia.org/wiki/DNA_end
  • en.wikipedia.org/wiki/Supernatant
  • en.wikipedia.org/wiki/ABTS
  • en.wikipedia.org/wiki/Conserved_sequence
  • en.wikipedia.org/wiki/Peptide_mass_fingerprinting
  • en.wikipedia.org/wiki/Dithiothreitol
  • en.wikipedia.org/wiki/Uranyl_acetate


and many more Sciencetalks (talk) —Preceding comment was added at 03:13, 4 January 2008 (UTC)


[edit] Lack of References/Sources

I noticed that not only is this article relatively short, it also cites no sources.

Works of Sweat (talk) 23:26, 26 March 2008 (UTC)