Far-Eastern blot

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Far-Eastern blotting is a technique developed in the 1990s by T. Taki and colleagues[1] at the Cellular Technology Institute of Otsuka Pharmaceutical Co., Japan for the analysis of lipids separated by high-performance thin layer chromatography (HPTLC). The lipids are transferred from the HPTLC plate to a PVDF membrane for futher analysis, for example by enzymatic or ligand binding assays[1] or mass spectrometry[2].

[edit] References

  1. ^ a b D. Ishikawa and T. Taki (1998), “Micro-scale analysis of lipids by far-eastern blot (TLC blot)”, Nihon yukagaku kaishi 47 (10): 963-970, <http://cat.inist.fr/?aModele=afficheN&cpsidt=1638632> 
  2. ^ Hamasaki H, Aoyagi M, Kasama T, Handa S, Hirakawa K, Taki T (January 1999). "GT1b in human metastatic brain tumors: GT1b as a brain metastasis-associated ganglioside". Biochim. Biophys. Acta 1437 (1): 93–9. PMID 9931455. 

[edit] See also

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