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An exopeptidase is an enzyme produced in the pancreas that catalyses the removal of an amino acid from the end of a polypeptide chain. Used for N-terminus or C-terminus determination of a protein. Exopeptidase cleaves the end of a polypeptide chain. Aminopeptidase, an enzyme in the brush border of the small intestine, will cleave a single amino acid from the aminoterminal. Carboxypeptidase, which is a digestive enzyme present in pancreatic juice, will cleave a single amino acid from the carboxylic end of the peptide.
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