Talk:ELISA
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[edit] ELISA
ELISA and EIA are closley related, but different things - ELISA is a swedish invention and EIA is a Dutch invention
The article is completely impenetrable to laypersons. Not only are the explanations immersed in jargon, but there is no plainly stated purpose of ELISA. Why would one use it? What for? Are there newer techniques that (may) make it obsolete?
Does anyone have information on "false positive" and "false negative" rates and interpretations? What are these rates? What situations can lead to a false positive or a false negative ... both test errors as well as clinical causes.
- I suspect that false postives and negatives are related to the specific test being run by ELISA, rather than the general technique. I think that that individual reagents and their conditions are where problems would mostly arise from. Dancarney 22:44, 11 July 2006 (UTC)
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- You are correct. Not only that, but false positive and false negative rates are also dependent upon who manufactured th kit, what batch the kit came from, and a large number of other factors. Considering that the antibodies used in ELISA usually come from injecting rabbits or goats and collecting the antibodies, quality control c
[edit] ELISA-sandwich.svg
This picture is confusing and do not correspond to the written description of a sandwich ELISA. It looks like a mixture between a indirect and sandwich ELISA. I think the enzyme-linked antibody binds to the antigen directly. No "detecting antibody" is needed, so the third step described in the picture, "(3) detecting antibody is added, and binds to antigen;" is wrong and should be deleted. /Izor 130.243.248.239 17:08, 15 October 2006 (UTC)
- I agree that the text and the image do not match up. I think the image actually represents a very common methodology used for ELISA (at least in my experience). You're right that a detecting antibody may not be needed if the secondary antibody is enzyme-conjugated, but in practice the conjugation is expensive and thus can be much more practically done by using an extra step with a detecting antibody. Anyway, I've been meaning to fix up and clarify the text for a while, but I haven't had time to do it. Please go ahead if you have the time, though...Be bold! — JVinocur (talk • contribs) 17:30, 16 October 2006 (UTC)
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- I've attempted to make this clearer. If someone would like to rewrite so that it reads more nicely, they could then delete these three posts. --Username132 (talk) 18:15, 24 October 2006 (UTC)
I've printed off this article to refer to with a university project I'm doing, however the image prints off with a black background, and all that shows is the blue and red shapes. I've tried saving the image and changing it to jpeg or similar, but same happens? Is there any reason for this? can anyone help?! Thanks Alex 17:12, 24 November 2006 (UTC)
- Not sure what to tell you, unfortunately...you might try using a different web browser, I guess? — JVinocur (talk • contribs) 23:44, 26 November 2006 (UTC)
- It's OK now, I used the ingenious, and extremely technical technique of pressing 'print screen' to get a screen shot of the image! Thanks for your help anyway, it probably is my browser; I use firefox. Alex 23:29, 10 December 2006 (UTC)
[edit] precautions while doing ELISA
tell me the precautions
Response to Precautions: What exactly are you looking for in terms of precautionary steps? Safety? Potential sources of error? That's a tad vague there. —Preceding unsigned comment added by 69.123.232.139 (talk) 15:34, 8 September 2007 (UTC)
[edit] RIA
I changed this:
Prior to the development of the EIA/ELISA, immunoassays were conducted using radioactively-labeled antigens or antibodies in a technique called radioimmunoassay.
To this:
Prior to the development of the EIA/ELISA, the only option for conducting an immunoassay was radioimmunoassay, a technique using radioactively-labeled antigens or antibodies.
The original wording implied that RIA is no longer used, which is inaccurate. 152.133.6.130 21:10, 26 October 2007 (UTC)
[edit] WikiProject class rating
This article was automatically assessed because at least one WikiProject had rated the article as start, and the rating on other projects was brought up to start class. BetacommandBot 16:25, 10 November 2007 (UTC)
[edit] Applications
I made a couple of changes to the "Applications" section for your perusal. I'm not sure I did the best job of wording the changes, so any comments and/or edits would be appreciated.
1) I added one application of ELISA -- specifically, its use in toxicology.
2) I added a paragraph detailing one method of establishing a cutoff.
--206.194.127.112 (talk) 22:48, 14 May 2008 (UTC)
- Hi 206.194.127.112, and thanks for the edit. I'm no expert on ELISA but it looked like a good contribution. I made a small copyedit to make the cutoff interpretation a little easier to follow. Keep up the good work! Adrian J. Hunter(talk•contribs) 05:37, 24 May 2008 (UTC)
