Image:ELISA-sandwich.svg

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ELISA-sandwich.svg‎ (SVG file, nominally 640 × 320 pixels, file size: 24 KB)

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Description

A sandwich ELISA. (1) Plate is coated with a capture antibody; (2) sample is added, and any antigen present binds to capture antibody; (3) detecting antibody is added, and binds to antigen; (4) enzyme-linked secondary antibody is added, and binds to detecting antibody; (5) substrate is added, and is converted by enzyme to detectable form.
Source

Own work
Date

Designed 2006-05-08
Author

Jeffrey M. Vinocur
Permission
(Reusing this image)

Own work, attribution required (Multi-license with GFDL and Creative Commons CC-BY 2.5)

I, the copyright holder of this work, hereby publish it under the following licenses:
GNU head Permission is granted to copy, distribute and/or modify this document under the terms of the GNU Free Documentation license, Version 1.2 or any later version published by the Free Software Foundation; with no Invariant Sections, no Front-Cover Texts, and no Back-Cover Texts. A copy of the license is included in the section entitled "GNU Free Documentation license".

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Creative Commons Attribution icon		 This file is licensed under Creative Commons Attribution 2.5 License
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File history

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Date/TimeDimensionsUserComment
current15:39, 9 May 2006640×320 (24 KB)JVinocur ({{Information| |Description='''A sandwich ''ELISA'''''''. (1) Plate is coated with a capture antibody; (2) sample is added, and any antigen present binds to capture antibody; (3) detecting antibody is added, and binds to antigen; (4) enzyme-linked second)
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