Electron tomography
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Electron Tomography (ET) is a tomography technique for obtaining detailed 3D structures of macromolecular objects. In the process, a beam of electrons is passed through the sample at incremental degrees of rotation around the center of the target sample. As part of an electron microscope, this information is collected and used to assemble a three dimensional image of the target. Current resolutions of ET systems are in the 5-20 nm range, suitable for examining supra-molecular multi-protein structures, although not the secondary and tertiary structure of an individual protein or polypeptide.
