Ehrlich's reagent

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Ehrlich's reagent (p-dimethyl-aminobenzaldehyde dissolved in 20% HCL). It is used for detection of urobilinogen in fresh cool urine.

If urine sample is left to oxidize in air to form urobilin the reagent will not detect the urobilinogen.

By adding few drops of reagent to 3 ml of urine in a test tube one can see a change of color from above, to dark pink or red. The degree of color change is proportional to the amount of urobilinogen in the urine sample.