Ecdysone 20-monooxygenase

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In enzymology, an ecdysone 20-monooxygenase (EC 1.14.99.22) is an enzyme that catalyzes the chemical reaction

ecdysone + AH₂ + O₂ $\rightleftharpoons$ 20-hydroxyecdysone + A + H₂O

The 3 substrates of this enzyme are ecdysone, AH2, and O₂, whereas its 3 products are 20-hydroxyecdysone, A, and H₂O.

This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derive from O miscellaneous. The systematic name of this enzyme class is Ecdysone,hydrogen-donor:oxygen oxidoreductase (20-hydroxylating). Other names in common use include alpha-ecdysone C-20 hydroxylase, and ecdysone 20-hydroxylase.

[edit] References

IUBMB entry for 1.14.99.22
BRENDA references for 1.14.99.22 (Recommended.)
PubMed references for 1.14.99.22
PubMed Central references for 1.14.99.22
Google Scholar references for 1.14.99.22
Johnson P, Rees HH (1977). "The mechanism of C-20 hydroxylation of alpha-ecdysone in the desert locust, Schistocerca gregaria". Biochem. J. 168: 513–20. PMID 606249.
Nigg HN, Svoboda JA, Thompson MJ, Dutky SR, Kaplanis JN, Robbins WE (1976). "Ecdysome 20-hydroxylase from the midgut of the tobacco hornworm (Manduca sexta L.)". Experientia. 32: 438–9. doi:10.1007/BF01920781. PMID 5286.
Gilbert LI (1979). "Ecdysone 20-monooxygenase: characterization of an insect cytochrome p-450 dependent steroid hydroxylase". Mol. Cell. Endocrinol. 15: 111–33. doi:10.1016/0303-7207(79)90033-9. PMID 488526.