DRAP1
From Wikipedia, the free encyclopedia
DR1-associated protein 1 (negative cofactor 2 alpha)
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PDB rendering based on 1jfi. | ||||||||||||||
Available structures: 1jfi | ||||||||||||||
Identifiers | ||||||||||||||
Symbol(s) | DRAP1; NC2-alpha | |||||||||||||
External IDs | OMIM: 602289 MGI: 1913806 HomoloGene: 4703 | |||||||||||||
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RNA expression pattern | ||||||||||||||
Orthologs | ||||||||||||||
Human | Mouse | |||||||||||||
Entrez | 10589 | 66556 | ||||||||||||
Ensembl | ENSG00000175550 | ENSMUSG00000024914 | ||||||||||||
Uniprot | Q14919 | Q4FJW2 | ||||||||||||
Refseq | NM_006442 (mRNA) NP_006433 (protein) |
XM_976049 (mRNA) XP_981143 (protein) |
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Location | Chr 11: 65.44 - 65.45 Mb | Chr 19: 5.42 - 5.42 Mb | ||||||||||||
Pubmed search | [1] | [2] |
DR1-associated protein 1 (negative cofactor 2 alpha), also known as DRAP1, is a human gene.[1]
Transcriptional repression is a general mechanism for regulating transcriptional initiation in organisms ranging from yeast to humans. Accurate initiation of transcription from eukaryotic protein-encoding genes requires the assembly of a large multiprotein complex consisting of RNA polymerase II and general transcription factors such as TFIIA, TFIIB, and TFIID. DR1 is a repressor that interacts with the TATA-binding protein (TBP) of TFIID and prevents the formation of an active transcription complex by precluding the entry of TFIIA and/or TFIIB into the preinitiation complex. The protein encoded by this gene is a corepressor of transcription that interacts with DR1 to enhance DR1-mediated repression. The interaction between this corepressor and DR1 is required for corepressor function and appears to stabilize the TBP-DR1-DNA complex.[1]
[edit] References
[edit] Further reading
- Mermelstein F, Yeung K, Cao J, et al. (1996). "Requirement of a corepressor for Dr1-mediated repression of transcription.". Genes Dev. 10 (8): 1033–48. PMID 8608938.
- Goppelt A, Stelzer G, Lottspeich F, Meisterernst M (1996). "A mechanism for repression of class II gene transcription through specific binding of NC2 to TBP-promoter complexes via heterodimeric histone fold domains.". EMBO J. 15 (12): 3105–16. PMID 8670811.
- Yeung K, Kim S, Reinberg D (1997). "Functional dissection of a human Dr1-DRAP1 repressor complex.". Mol. Cell. Biol. 17 (1): 36–45. PMID 8972183.
- Ikeda K, Halle JP, Stelzer G, et al. (1998). "Involvement of negative cofactor NC2 in active repression by zinc finger-homeodomain transcription factor AREB6.". Mol. Cell. Biol. 18 (1): 10–8. PMID 9418848.
- Castaño E, Gross P, Wang Z, et al. (2000). "The C-terminal domain-phosphorylated IIO form of RNA polymerase II is associated with the transcription repressor NC2 (Dr1/DRAP1) and is required for transcription activation in human nuclear extracts.". Proc. Natl. Acad. Sci. U.S.A. 97 (13): 7184–9. doi: . PMID 10852970.
- Kamada K, Shu F, Chen H, et al. (2001). "Crystal structure of negative cofactor 2 recognizing the TBP-DNA transcription complex.". Cell 106 (1): 71–81. PMID 11461703.
- Iratni R, Yan YT, Chen C, et al. (2003). "Inhibition of excess nodal signaling during mouse gastrulation by the transcriptional corepressor DRAP1.". Science 298 (5600): 1996–9. doi: . PMID 12471260.
- Denko N, Wernke-Dollries K, Johnson AB, et al. (2003). "Hypoxia actively represses transcription by inducing negative cofactor 2 (Dr1/DrAP1) and blocking preinitiation complex assembly.". J. Biol. Chem. 278 (8): 5744–9. doi: . PMID 12477712.
- Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi: . PMID 12477932.
- Leonard D, Ajuh P, Lamond AI, Legerski RJ (2003). "hLodestar/HuF2 interacts with CDC5L and is involved in pre-mRNA splicing.". Biochem. Biophys. Res. Commun. 308 (4): 793–801. PMID 12927788.
- Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).". Genome Res. 14 (10B): 2121–7. doi: . PMID 15489334.
- Klejman MP, Pereira LA, van Zeeburg HJ, et al. (2004). "NC2alpha interacts with BTAF1 and stimulates its ATP-dependent association with TATA-binding protein.". Mol. Cell. Biol. 24 (22): 10072–82. doi: . PMID 15509807.
- Lewis BA, Sims RJ, Lane WS, Reinberg D (2005). "Functional characterization of core promoter elements: DPE-specific transcription requires the protein kinase CK2 and the PC4 coactivator.". Mol. Cell 18 (4): 471–81. doi: . PMID 15893730.
- Stelzl U, Worm U, Lalowski M, et al. (2005). "A human protein-protein interaction network: a resource for annotating the proteome.". Cell 122 (6): 957–68. doi: . PMID 16169070.
- Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network.". Nature 437 (7062): 1173–8. doi: . PMID 16189514.
- Assmann EM, Alborghetti MR, Camargo ME, Kobarg J (2006). "FEZ1 dimerization and interaction with transcription regulatory proteins involves its coiled-coil region.". J. Biol. Chem. 281 (15): 9869–81. doi: . PMID 16484223.
- Lim J, Hao T, Shaw C, et al. (2006). "A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration.". Cell 125 (4): 801–14. doi: . PMID 16713569.
- Albert TK, Grote K, Boeing S, et al. (2007). "Global distribution of negative cofactor 2 subunit-alpha on human promoters.". Proc. Natl. Acad. Sci. U.S.A. 104 (24): 10000–5. doi: . PMID 17548813.