Talk:DNA electrophoresis

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This seems to be a duplicate, or at least overlap considerably, of gel electrophoresis, and perhaps should be merged. There should be single major overview article on electrophoresis and subarticles, but at the moment they are somewhat fragmented. --Lexor|Talk 12:02, Dec 2, 2004 (UTC)

I do not think that a merge is needed for the DNA gel electrophoresis page, but a removal of information that is already covered in the Gel electrophoresis article should be removed. DNA electrophoresis is extremely important in molecular biology apart from the other types of electrophoresis. I was hoping to be able to add information in this article regarding the applications of DNA electrophoresis, including PCR analysis and restriction map digests. A merge is definitely not warranted. --G3pro 14:45, 2 Dec 2004 (UTC)

polilipo

I agree with polilipo. I think there needs to be some shifts with information between the Gel Electrophoresis page and this page because they are two different techniques, despite the fact that they are related. Neagley 02:27, 1 June 2007 (UTC)

You guys want to combine, I think this page needs to be expanded, greatly.

Electrophoresis is a broad category in which gel electrophoresis is a subset. Within that subset DNA electrophoresis is another subset, and Agarose gel electrophoresis is another subset. Since this page deals with DNA electrophoresis I find that it needs to be expanded

• Section - agarose gel electrophoresis
  • Subsection - standard agarose gel electrophoresis of DNA
    • Sub-subsection - Relative mobility of linear DNA (DS and SS DNA), circular nicked DNA, supercoiled DNA.
  • Subsection - Variable feild gel electrophoresis.
  • Subsection - Low melting point agarose
  • Subsection - Purifying DNA from agarose gels.
• Section 2 - Acrylamide gel electrophoresis

It would also be nice to have a section on improving resolution, such as current ramping and max current versus fragment size, etc. Also on tracking dyes and relative mobility in different percentage gels. How to make a tracking dye buffer would be nice. Pdeitiker 14:06, 25 August 2007 (UTC)