Deacetylcephalosporin-C acetyltransferase

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In enzymology, a deacetylcephalosporin-C acetyltransferase (EC 2.3.1.175) is an enzyme that catalyzes the chemical reaction

acetyl-CoA + deacetylcephalosporin C $\rightleftharpoons$ CoA + cephalosporin C

Thus, the two substrates of this enzyme are acetyl-CoA and deacetylcephalosporin C, whereas its two products are CoA and cephalosporin C.

This enzyme belongs to the family of transferases, specifically those acyltransferases transferring groups other than aminoacyl groups. The systematic name of this enzyme class is acetyl-CoA:deacetylcephalosporin-C O-acetyltransferase. Other names in common use include acetyl-CoA:deacetylcephalosporin-C acetyltransferase, DAC acetyltransferase, cefG, deacetylcephalosporin C acetyltransferase, acetyl coenzyme A:DAC acetyltransferase, acetyl-CoA:DAC acetyltransferase, CPC acetylhydrolase, acetyl-CoA:DAC O-acetyltransferase, and DAC-AT. This enzyme participates in penicillin and cephalosporin biosynthesis.

[edit] References

IUBMB entry for 2.3.1.175
BRENDA references for 2.3.1.175 (Recommended.)
PubMed references for 2.3.1.175
PubMed Central references for 2.3.1.175
Google Scholar references for 2.3.1.175
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Gutierrez S, Velasco J, Fernandez FJ, Martin JF (1992). "The cefG gene of Cephalosporium acremonium is linked to the cefEF gene and encodes a deacetylcephalosporin C acetyltransferase closely related to homoserine O-acetyltransferase". J. Bacteriol. 174: 3056–64. PMID 1569032.
K (1992). "Cloning and disruption of the cefG gene encoding acetyl coenzyme A: deacetylcephalosporin C o-acetyltransferase from Acremonium chrysogenum". Biochem. Biophys. Res. Commun. 186: 40–6. doi:10.1016/S0006-291X(05)80772-7. PMID 1632779.
JL, Diez B, Martin JF (1997). "Expression of the cefG gene is limiting for cephalosporin biosynthesis in Acremonium chrysogenum". Appl. Microbiol. Biotechnol. 48: 606–14. doi:10.1007/s002530051103. PMID 9421924.
Velasco J, Gutierrez S, Campoy S, Martin JF (Pt 3). "Molecular characterization of the Acremonium chrysogenum cefG gene product: the native deacetylcephalosporin C acetyltransferase is not processed into subunits". Biochem. J. 337: 379–85. doi:10.1042/0264-6021:3370379. PMID 9895280.
AT, Kosalkova K (1994). "Expression of genes and processing of enzymes for the biosynthesis of penicillins and cephalosporins". Antonie. Van. Leeuwenhoek. 65: 227–43. doi:10.1007/BF00871951. PMID 7847890.