Deacetoxycephalosporin-C synthase

From Wikipedia, the free encyclopedia

In enzymology, a deacetoxycephalosporin-C synthase (EC 1.14.20.1) is an enzyme that catalyzes the chemical reaction

penicillin N + 2-oxoglutarate + O₂ $\rightleftharpoons$ deacetoxycephalosporin C + succinate + CO₂ + H₂O

The 3 substrates of this enzyme are penicillin N, 2-oxoglutarate, and O₂, whereas its 4 products are deacetoxycephalosporin C, succinate, CO₂, and H₂O.

This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with 2-oxoglutarate as one donor, and the other dehydrogenated. The systematic name of this enzyme class is penicillin-N,2-oxoglutarate:oxygen oxidoreductase (ring-expanding). Other names in common use include DAOCS, penicillin N expandase, and DAOC synthase. This enzyme participates in penicillin and cephalosporin biosynthesis.

1 Structural studies
2 References
3 External links
- 3.1 Gene Ontology (GO) codes

[edit] Structural studies

As of late 2007, 7 structures have been solved for this class of enzymes, with PDB accession codes 1UNB, 1UO9, 1UOB, 1UOF, 1UOG, 1W28, and 1W2A.

[edit] References

IUBMB entry for 1.14.20.1
BRENDA references for 1.14.20.1 (Recommended.)
PubMed references for 1.14.20.1
PubMed Central references for 1.14.20.1
Google Scholar references for 1.14.20.1
Cantwell C, Beckmann R, Whiteman P, Queener SW, Abraham EP (1992). "Isolation of deacetoxycephalosporin C from fermentation broths of Penicillium chrysogenum transformants: construction of a new fungal biosynthetic pathway". Proc. R. Soc. Lond. B. Biol. Sci. 248: 283–9. doi:10.1098/rspb.1992.0073. PMID 1354366.
Lee HJ, Lloyd MD, Harlos K, Clifton IJ, Baldwin JE, Schofield CJ (2001). "Kinetic and crystallographic studies on deacetoxycephalosporin C synthase (DAOCS)". J. Mol. Biol. 308: 937–48. doi:10.1006/jmbi.2001.4649. PMID 11352583.
Yeh WK, Ghag SK and Queener SW (1992). "Enzymes for epimerization of isopenicillin N, ring expansion of penicillin N, and 3'-hydroxylation of deacetoxycephalosporin C Function, evolution, refolding, and enzyme engineering". Ann. N.Y. Acad. Sci. 672: 396–408.
Andersson I (1998). "Structure of a cephalosporin synthase". Nature. 394: 805–9. doi:10.1038/29575. PMID 9723623.
Dotzlaf JE, Yeh WK (1989). "Purification and properties of deacetoxycephalosporin C synthase from recombinant Escherichia coli and its comparison with the native enzyme purified from Streptomyces clavuligerus". J. Biol. Chem. 264: 10219–27. PMID 2656705.